Description

3-Bromo-3-(4-Nitrophenyl)-3H-Diazirine is a specialized photoaffinity labeling reagent, a crucial tool for probing biomolecular interactions and mapping binding sites. Its core value lies in generating highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking of ligands to their target proteins or nucleic acids. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, and proteomics who require precise molecular mapping for drug design and mechanism-of-action studies.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalent capture of transient or weak protein-ligand interactions in drug target identification.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize enzyme active sites and off-targets.
• Bioconjugation & Probe Development: Serves as a photoreactive handle for synthesizing functionalized probes, such as biotinylated or fluorescent-tagged derivatives.
• Structural Biology Studies: Assists in elucidating three-dimensional binding pockets and molecular recognition events.
• Material Science Surface Modification: Potential use in creating covalently attached functional layers on surfaces for biosensor development.

Basic Information

Product Name	3-Bromo-3-(4-Nitrophenyl)-3H-Diazirine
CAS No.	115127-49-6
Molecular Formula	C8H5BrN3O2
Molecular Weight	257.05 g/mol
Synonyms	3-Bromo-3-(4-nitrophenyl)diazirine; 4-Nitrophenyl 3-bromodiazirine; p-Nitrophenyl 3-bromodiazirine; 3-Bromo-3-(p-nitrophenyl)diazirine; 3H-Diazirine, 3-bromo-3-(4-nitrophenyl)-; Diaziridine, 3-bromo-3-(4-nitrophenyl)-; Photoaffinity Labeling Reagent 115127-49-6
EINECS	Contact for details

Quality Control

Our 3-Bromo-3-(4-Nitrophenyl)-3H-Diazirine is produced and handled under strict quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification and spectroscopic methods (NMR, IR) for structural confirmation. A Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere. The container should be kept in the original packaging and allowed to equilibrate to room temperature before opening to minimize condensation. Keep away from heat and sources of ignition.

Specification

Item	Specification
Appearance	Yellow to orange crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Melting Point	Contact for details
Solubility	Soluble in common organic solvents (DMSO, DMF, Acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.