Description

3-(Trifluoromethyl)-3-(3-Hydroxyphenyl)Diazirine is a specialized diazirine-based photoaffinity labeling reagent, a critical tool for probing biomolecular interactions. Its unique structure, featuring a reactive carbene precursor and a phenolic handle, enables covalent cross-linking upon UV irradiation for target identification and mapping studies in complex biological systems. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, and proteomics who require high-purity, reliable probes for advanced binding site analysis.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalent capture and identification of drug targets and protein-ligand interaction sites.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to study enzyme function and discover novel therapeutic targets.
• Bioconjugation & Probe Synthesis: The phenolic hydroxyl group allows for derivatization and attachment to various scaffolds, beads, or reporter tags.
• Structural Biology Studies: Aids in elucidating the structure and dynamics of macromolecular complexes through cross-linking mass spectrometry.
• Materials Science Research: Potential application in surface functionalization and creating photo-reactive polymeric materials.

Basic Information

Product Name	3-(Trifluoromethyl)-3-(3-Hydroxyphenyl)Diazirine
CAS No.	113787-85-2
Molecular Formula	C9H5F3N2O
Molecular Weight	214.15 g/mol
Synonyms	3-(3-Hydroxyphenyl)-3-(trifluoromethyl)diazirine; 3-Hydroxy-3'-(trifluoromethyl)phenyldiazirine; 3-(Trifluoromethyl)-3-(3-hydroxyphenyl)diaziridine; Photoaffinity Labeling Reagent 113787-85-2; 3-(3-Hydroxyphenyl)-3-(trifluoromethyl)diazacyclopropene; TFD-hydroxyphenyl
EINECS	Contact for details

Quality Control

We maintain stringent quality control protocols for our photoaffinity labeling reagents. Each batch of 3-(Trifluoromethyl)-3-(3-Hydroxyphenyl)Diazirine is analyzed to ensure high chemical purity and functional integrity, critical for reproducible research outcomes. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. Allow the container to reach room temperature before opening to minimize moisture condensation. Keep under an inert atmosphere for long-term storage to maximize stability.

Specification

Item	Specification
Appearance	Off-white to light yellow crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	< 0.5%
Solubility	Soluble in common organic solvents (DMSO, DMF, Acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.