Description

Nitro-Paps CAS NO 113516-70-4 is a specialized chromogenic substrate widely used in clinical diagnostics and biochemical research. Its primary value lies in its high sensitivity and specificity for detecting alkaline phosphatase (ALP) activity, enabling accurate and reliable enzymatic assays. This compound is essential for manufacturers and research laboratories developing diagnostic kits for liver function, bone disorders, and other clinical conditions.

Application

• Clinical Diagnostic Kits: As a key reagent in enzymatic assays for measuring Alkaline Phosphatase (ALP) levels in serum, a critical marker for liver and bone health.
• Biochemical Research: For studying enzyme kinetics, inhibition, and the activity of alkaline phosphatase in various experimental models.
• Immunoassay Development: Used in ELISA (Enzyme-Linked Immunosorbent Assay) and other immunoassays where ALP is employed as an enzyme label.
• Point-of-Care Testing (POCT): Formulated into dry chemistry strips or cartridges for rapid, on-site diagnostic devices.
• Pharmaceutical Quality Control: Assaying enzymatic activity in drug development and biomanufacturing processes.
• Veterinary Diagnostics: Adapted for use in animal health testing panels.

Basic Information

Product Name	Nitro-Paps
CAS No.	113516-70-4
Molecular Formula	C16H16N4O9PS
Molecular Weight	480.36 g/mol
Synonyms	2-Chloro-5-(4-Nitrophenoxy)phenyl Phosphate Disodium Salt; PNPP Derivative; 4-Nitrophenyl Phosphate (PNPP) Analog; Alkaline Phosphatase Substrate PNPP-Nitro; Nitrophenylphosphate Substrate; 5-Chloro-2-((4-nitrophenoxy)phenyl) Dihydrogen Phosphate; Diagnostic Reagent ALP Substrate
EINECS	Contact for details

Quality Control

Our Nitro-Paps is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive diagnostic applications. Each lot is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the stringent requirements of the in vitro diagnostic (IVD) industry. Comprehensive Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	95.0 - 105.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in buffer
Functional Performance (ALP Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.