Description

n-(3-Sulfopropyl)-3 3' 5 5'-Tetramethylbenzidine is a specialized sulfonated derivative of the chromogenic substrate 3,3',5,5'-Tetramethylbenzidine (TMB). This compound is engineered to offer enhanced water solubility and stability, making it a critical reagent in advanced diagnostic and analytical applications. It is primarily required by manufacturers and research institutions in the life sciences, in-vitro diagnostics (IVD), and biotechnology sectors for developing sensitive and reliable detection systems.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): As a soluble, chromogenic substrate for horseradish peroxidase (HRP) in high-sensitivity diagnostic kits.
• Lateral Flow Assays (LFAs): Used in rapid test strips for point-of-care diagnostics, including infectious disease and pregnancy tests.
• Immunohistochemistry (IHC): Employed as a detection reagent for visualizing antigen-antibody interactions in tissue samples.
• Biosensor Development: Integral to the fabrication of electrochemical and optical biosensors for medical and environmental monitoring.
• Molecular Biology Research: As a key component in blotting techniques (e.g., Western, Southern) for protein and nucleic acid detection.
• Clinical Chemistry Analyzers: A standardized reagent in automated systems for quantifying specific biomarkers.

Basic Information

Product Name	n-(3-Sulfopropyl)-3 3' 5 5'-Tetramethylbenzidine
CAS No.	102062-46-4
Molecular Formula	C22H32N2O3S
Molecular Weight	404.57 g/mol
Synonyms	TMB-S; 3,3',5,5'-Tetramethylbenzidine-N-(3-sulfopropyl) Salt; Sulfopropyl TMB; TMB Sulfonate; N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine; TMBPS; Chromogenic Peroxidase Substrate TMB-S; Water-soluble TMB
EINECS	Contact for details

Quality Control

Our n-(3-Sulfopropyl)-3 3' 5 5'-Tetramethylbenzidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) detailing purity, absorbance characteristics, and performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Absorbance (λmax)	370 nm & 652 nm (in solution)
Water Content (KF)	≤ 2.0%
Solubility	Freely soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.