Description

Thiourea, N-6-O-(N-acetyl-.alpha.-neuraminosyl)-(23)-O-.beta.-D-galactopyranosyl-(14)-2-(acetylamino)-2-deoxy-.beta.-D-glucopyranosyloxyhexyl-N-(3,6-dihydroxy-3-oxospiroisobenzofuran-1(3H),9-9Hxanthen-5-yl)-, disodium salt is a highly specialized, fluorescent-labeled glycosyl thiourea derivative designed for advanced biochemical research and diagnostic applications. This compound serves as a critical tool for the specific detection, labeling, and study of carbohydrate-protein interactions, particularly those involving sialic acid residues. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers engaged in glycobiology, assay development, and high-sensitivity detection systems.

Application

• Glycobiology Research: As a fluorescent probe for studying lectin binding, glycan recognition, and cell surface carbohydrate dynamics.
• Diagnostic Assay Development: A key component in the design of sensitive fluorescence-based immunoassays (FLISA) and lateral flow tests for pathogen or biomarker detection.
• Bioconjugation & Labeling: Used for the site-specific fluorescent labeling of antibodies, proteins, or nanoparticles via its reactive thiourea and glycosyl groups.
• High-Content Screening (HCS): Employed in automated cellular imaging systems to visualize and quantify specific carbohydrate-mediated processes.
• Flow Cytometry: Acts as a staining reagent for the analysis of cell surface glycosylation patterns using flow cytometers.
• Microarray Technology: Printed onto glycan microarrays to profile antibody or lectin specificity in a high-throughput format.
• Drug Discovery: Used in target validation and screening assays for inhibitors of glycosyltransferases or carbohydrate-binding proteins.
• Academic & Pharmaceutical R&D: Fundamental research tool for investigating the role of complex carbohydrates in disease mechanisms, including cancer and immunology.

Basic Information

Product Name	Thiourea, N-6-O-(N-acetyl-.alpha.-neuraminosyl)-(23)-O-.beta.-D-galactopyranosyl-(14)-2-(acetylamino)-2-deoxy-.beta.-D-glucopyranosyloxyhexyl-N-(3,6-dihydroxy-3-oxospiroisobenzofuran-1(3H),9-9Hxanthen-5-yl)-, disodium salt
CAS No.	184294-05-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Fluorescein-labeled Sialyl Lactosamine Thiourea; Sialyl Lewis X Fluorescein Conjugate (analog); Glycosyl Fluorescein Thiourea Derivative; Fluorescent Glycoconjugate Probe; 5(6)-FITC labeled Sialyllactosamine derivative; Disodium 5-(and-6)-carboxyfluorescein-sialyllactosamine thiourea; N-(Fluorescein-5-thiocarbamoyl)-sialyllactosamine, disodium salt; Sialyllactosamine-FITC
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under controlled conditions to ensure batch-to-batch consistency for critical research applications. Quality is verified through a comprehensive suite of analytical techniques including HPLC for purity, mass spectrometry (MS) for identity confirmation, and NMR for structural validation. A detailed Certificate of Analysis (COA) documenting identity, purity (HPLC assay), fluorescence properties, and absence of critical impurities is provided with each batch.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area Percent)
Assay (Spectrophotometry)	≥ 90% (on dried basis)
Water Content (KF)	≤ 5.0 %
Fluorescence Properties	Ex/Em: ~494 nm / ~518 nm (in suitable buffer)
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.