Description

Blue Dextran For Gel Filtration is a high-molecular-weight polysaccharide derivative, covalently bound to the chromophore Reactive Blue 2. This product is specifically designed and purified for use as a void volume marker in gel filtration (size-exclusion) chromatography. Its distinct blue color and high molecular weight make it an essential tool for calibrating columns and determining the exclusion limits of various separation media. It is a critical reagent for researchers and quality control laboratories in the biotechnology, pharmaceutical, and academic research sectors who require precise molecular size analysis of proteins, nucleic acids, and other macromolecules.

Application

• Primary calibration standard for determining the void volume (V0) in gel filtration chromatography systems.
• Tool for column performance qualification and validation in biopharmaceutical manufacturing and research.
• Molecular weight marker for estimating the exclusion limits of Sephadex, Sepharose, and other gel filtration media.
• Used in the study of protein aggregation, polymer characterization, and macromolecular complex analysis.
• Teaching and demonstration aid in academic laboratories for principles of size-exclusion chromatography.
• Process development in downstream purification workflows for biologics.

Basic Information

Item	Detail
Product Name	Blue Dextran For Gel Filtration
CAS No.	87915-38-6
Molecular Formula	(C6H10O5)n·C23H27ClN6O13S4
Molecular Weight	~2,000,000 (Dextran backbone, average)
Synonyms	Blue Dextran 2000; Cibacron Blue Dextran; Dextran Blue; Reactive Blue 2-Dextran Conjugate; Dextran-Cibacron Blue; Gel Filtration Marker Blue; Void Volume Marker Blue; Pharmacia Blue Dextran
EINECS	Contact for details

Quality Control

Our Blue Dextran For Gel Filtration is produced under a quality-focused system to ensure batch-to-batch consistency for critical chromatographic applications. Each lot is tested to confirm its performance as a high-molecular-weight exclusion marker. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as absorbance profile, solubility, and chromatographic performance. We support compliance needs for research and development under ISO 9001 quality management principles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Blue to dark blue powder or granules
Identification (IR)	Conforms to structure
Absorbance Maximum	~620-630 nm
Solubility	Soluble in water, saline; insoluble in organic solvents
Chromatographic Performance	Defined exclusion peak suitable for gel filtration calibration
Loss on Drying	< 10.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.