Description

p-Nitrophenyl 2-Acetamido-2-Deoxy-3,6-Di-o-Benzoyl--D-Glucopyranoside is a specialized glycoside derivative, primarily utilized as a chromogenic substrate in enzymatic assays. This compound is critical for research and development in biochemistry and molecular biology, enabling precise measurement of glycosidase activity. It is an essential reagent for pharmaceutical companies, diagnostic kit manufacturers, and academic research institutions focused on carbohydrate-active enzymes and related metabolic pathways.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for the detection and kinetic study of specific glycoside hydrolases, such as β-N-acetylglucosaminidases.
• Biochemical Research: Used in high-throughput screening (HTS) assays to identify enzyme inhibitors or activators for drug discovery programs.
• Diagnostic Reagent Manufacturing: Incorporated into diagnostic kits for the detection of enzyme deficiencies or specific biomarkers in clinical samples.
• Carbohydrate Chemistry Studies: Serves as a key intermediate or model compound in synthetic chemistry for studying glycosylation mechanisms and synthesizing complex oligosaccharides.
• Quality Control in Biologics: Employed in the characterization and potency testing of enzyme-based therapeutics to ensure batch-to-batch consistency.

Basic Information

Product Name	p-Nitrophenyl 2-Acetamido-2-Deoxy-3,6-Di-o-Benzoyl--D-Glucopyranoside
CAS No.	84564-22-7
Molecular Formula	C28H26N2O10
Molecular Weight	550.52 g/mol
Synonyms	PNP-β-GlcNAc-3,6-di-O-benzoyl; p-Nitrophenyl N-acetyl-3,6-di-O-benzoyl-β-D-glucosaminide; 4-Nitrophenyl 2-acetamido-2-deoxy-3,6-di-O-benzoyl-β-D-glucopyranoside; 2-Acetamido-2-deoxy-3,6-di-O-benzoyl-β-D-glucopyranosyl 4-nitrophenyl ether; β-D-Glucopyranoside, 2-(acetylamino)-2-deoxy-3,6-bis-O-(phenylcarbonyl)-, 4-nitrophenyl; Chromogenic substrate for β-N-acetylglucosaminidase; NAG-3,6-diOBz-pNP; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 2-Acetamido-2-Deoxy-3,6-Di-o-Benzoyl--D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product under inert atmosphere to maintain stability. The container should be kept tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.