Description

p-Nitrophenyl 4,6-Benzylidene-α-D-Glucopyranoside is a specialized glycoside derivative used as a critical chromogenic substrate in enzymatic assays. This compound is valued for its role in the precise measurement of α-glucosidase and related enzyme activities, providing researchers with a reliable tool for kinetic studies and inhibitor screening. It is primarily utilized by biochemical researchers, pharmaceutical developers, and diagnostic manufacturers working in enzymology and carbohydrate chemistry.

Application

• Enzymatic Substrate for α-Glucosidase: Acts as a chromogenic substrate for the spectrophotometric determination of α-glucosidase activity, where enzymatic hydrolysis releases p-nitrophenol, producing a measurable yellow color.
• Biochemical Research & Kinetic Studies: Employed in fundamental research to study enzyme kinetics, mechanisms, and inhibition constants for carbohydrate-active enzymes.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and evaluate potential inhibitors of α-glucosidase for therapeutic applications, such as in diabetes management.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for clinical and research laboratories to measure specific enzyme levels.
• Carbohydrate Chemistry Studies: Utilized as a model compound in synthetic and analytical chemistry to investigate glycoside hydrolysis and protective group chemistry.

Basic Information

Product Name	p-Nitrophenyl 4,6-Benzylidene-α-D-Glucopyranoside
CAS No.	83167-73-1
Molecular Formula	C19H19NO8
Molecular Weight	389.36 g/mol
Synonyms	4-Nitrophenyl 4,6-O-benzylidene-α-D-glucopyranoside; p-Nitrophenyl 4,6-O-Benzylidene-α-D-glucoside; PNPG-Bn; α-D-Glucopyranoside, 4,6-O-benzylidene-, 4-nitrophenyl; Benzylidene-protected p-Nitrophenyl α-D-glucoside; Chromogenic α-glucosidase substrate; 4,6-O-Benzylidene-α-D-glucopyranosyl 4-nitrobenzoate (related ester form); α-PNPG benzylidene acetal
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl 4,6-Benzylidene-α-D-Glucopyranoside is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The compound is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Specification available upon request
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.