Description

Mlu I CAS NO 81458-04-0 is a high-purity chemical compound, specifically a restriction endonuclease enzyme, essential for molecular biology research and biotechnological applications. Its primary value lies in its precise and reliable DNA cleavage activity at specific recognition sites, which is fundamental for genetic engineering, cloning, and genomic analysis. This product is a critical reagent for biotechnology companies, pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers requiring consistent enzymatic performance for nucleic acid manipulation.

Application

• Molecular Cloning: Essential for precise DNA digestion to create compatible ends for ligation in plasmid vector construction.
• Genetic Engineering: Used in recombinant DNA technology to insert or delete specific gene sequences.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: A key enzyme for DNA fingerprinting and genetic mapping studies.
• Diagnostic Assay Development: Employed in the manufacturing of diagnostic tests that rely on specific DNA sequence detection.
• Genomic Library Construction: Facilitates the fragmentation of genomic DNA into manageable segments for sequencing projects.
• Site-Directed Mutagenesis: Used in protocols to introduce specific mutations into DNA sequences for functional studies.

Basic Information

Item	Detail
Product Name	Mlu I
CAS No.	81458-04-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	MluI Restriction Enzyme; Endonuclease Mlu I; Restriction Endonuclease MluI; MluI (from Micrococcus luteus); 5'-A↓CGCGT-3'; Type II Restriction Enzyme MluI; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Mlu I is produced and tested under stringent quality management systems to ensure exceptional lot-to-lot consistency and optimal enzymatic activity. Each batch is rigorously analyzed for specific activity, purity, and the absence of non-specific nucleases and phosphatases. A comprehensive Certificate of Analysis (COA) detailing purity, activity units (U/µl), and storage buffer composition is provided with every shipment to guarantee it meets the exacting standards required for sensitive molecular biology applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer containing glycerol.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves λ DNA at the specific recognition site (5'-A↓CGCGT-3')
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Non-Specific Nuclease Activity	Not detectable
Phosphatase Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.