Description

Xba I is a high-purity restriction endonuclease enzyme widely used in molecular biology research and biotechnology applications. This product is essential for precise DNA manipulation, enabling researchers to perform accurate genetic analysis and construct recombinant DNA molecules. It is a critical reagent for laboratories in the pharmaceutical, academic, and diagnostic sectors engaged in genetic engineering, cloning, and genomic sequencing projects.

Application

• Molecular Cloning: Used for the specific digestion of DNA at the recognition sequence 5'-TCTAGA-3' to create compatible ends for ligation.
• Genetic Engineering: Essential for constructing recombinant plasmids, vectors, and other DNA constructs in synthetic biology.
• Genomic DNA Analysis: Employed in restriction fragment length polymorphism (RFLP) studies and genomic mapping.
• Diagnostic Assay Development: Used to prepare DNA samples and probes for various molecular diagnostic tests.
• Biopharmaceutical Production: A key tool in the research and development phase for producing biologics and gene therapies.
• Academic Research: Fundamental for university and institutional labs conducting basic research in genetics and molecular biology.

Basic Information

Product Name	Xba I
CAS No.	81295-42-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	N/A (Enzyme)
Synonyms	Endodeoxyribonuclease XbaI; XbaI Restriction Enzyme; Restriction Endonuclease XbaI; XbaI (from Xanthomonas badrii); EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Xba I is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot undergoes rigorous testing for specific activity, purity, and the absence of contaminating nucleases. Certificates of Analysis (COA) detailing purity, activity units, and storage buffer composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; handle on ice when thawed for use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Contaminating Activities	Absence of detectable exo- and endonuclease, phosphatase
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4 at 25°C
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.