Description

Bgl Ii CAS NO 81295-12-7 is a high-purity biochemical reagent widely recognized for its role as a restriction enzyme. This product is essential for precise DNA manipulation, offering reliable and consistent cutting at specific recognition sites. It is a critical component for molecular biology research, pharmaceutical development, and genetic engineering applications requiring high-fidelity DNA analysis and cloning.

Application

• Molecular Biology Research: Used for DNA restriction mapping, genotyping, and recombinant DNA technology.
• Genetic Engineering: Essential for gene cloning, plasmid construction, and the creation of DNA libraries.
• Diagnostic Development: Employed in the development of molecular diagnostic assays and kits for pathogen detection.
• Pharmaceutical R&D: Facilitates the study of genetic markers, gene function, and the production of biologics.
• Forensic Analysis: Applied in DNA fingerprinting and other forensic science techniques.
• Agricultural Biotechnology: Used in the development of genetically modified organisms (GMOs) and crop improvement studies.

Basic Information

Product Name	Bgl Ii
CAS No.	81295-12-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	BglII, Bgl II Restriction Endonuclease, BglII Enzyme, Endodeoxyribonuclease BglII, Restriction Enzyme BglII, BglII (Bacillus globigii), EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Bgl Ii is manufactured under stringent quality systems. Each lot undergoes rigorous testing for activity, specificity, and purity, ensuring it is free from contaminating nucleases and phosphatases. A comprehensive Certificate of Analysis (COA) detailing assay results, unit definition, and storage buffer composition is provided with every shipment to guarantee batch-to-batch consistency and performance reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C in the supplied storage buffer. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Activity (Units/µl)	≥ 10,000
Purity (SDS-PAGE)	≥ 95%
Specificity	Cleaves at AGATCT sequence
Contaminating Activities	Free of detectable exo- & endonucleases, RNase, and phosphatase
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.