Description

Rsa I is a high-purity biochemical reagent widely used in molecular biology research and diagnostic applications. Its primary value lies in its role as a specific restriction enzyme, enabling precise DNA manipulation and analysis for genetic studies. This product is essential for biotechnology companies, academic research laboratories, and pharmaceutical R&D departments involved in genomics, cloning, and molecular diagnostics.

Application

• Molecular Cloning: Used for the precise cleavage of DNA at specific recognition sites to facilitate gene insertion and vector construction.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: A key reagent for genetic fingerprinting and mutation detection in diagnostic and research settings.
• Genomic DNA Mapping: Employed in the analysis and mapping of complex genomes for research and biotechnological applications.
• Recombinant DNA Technology: Critical for the creation of recombinant DNA molecules in the development of biologics and gene therapies.
• Diagnostic Kit Manufacturing: Serves as a core component in the production of kits for genetic testing and pathogen detection.
• Academic & Industrial Research: Supports fundamental and applied research in genetics, synthetic biology, and metabolic engineering.

Basic Information

Product Name	Rsa I
CAS No.	80449-06-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Endonuclease RsaI; Restriction Enzyme Rsa I; RsaI restriction endonuclease; Deoxyribonuclease RsaI; RsaI (enzyme); EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Rsa I is manufactured under stringent quality systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is tested for specific activity, purity (free from non-specific nucleases and phosphatases), and stability. Certificates of Analysis (COA) detailing purity, activity units, and storage buffer composition are provided with every shipment to guarantee compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The product is supplied in a stabilized storage buffer; handle on ice when thawed for use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves pUC19 DNA at the recognized site
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Non-Specific Nuclease Activity	Not detected
Phosphatase Activity	Not detected
Storage Buffer	Tris-HCl, NaCl, EDTA, Glycerol, DTT

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.