Description

H2N-Peg2-Tbu CAS NO 756525-95-8 is a specialized bifunctional polyethylene glycol (PEG) derivative featuring a primary amine group and a tert-butyl ester-protected carboxylic acid, serving as a versatile heterobifunctional linker for bioconjugation and chemical synthesis. This compound is critical for creating stable, hydrophilic spacers in the development of advanced drug delivery systems, diagnostic reagents, and functional biomaterials. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors who require precise and reliable crosslinking agents.

Application

• Bioconjugation Linker: For coupling peptides, proteins, or antibodies to surfaces, nanoparticles, or other biomolecules via its amine-reactive and carboxyl-reactive ends.
• ADC (Antibody-Drug Conjugate) Development: As a key component in constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• PEGylation Reagent: To introduce hydrophilic PEG chains onto drugs or proteins, improving solubility, stability, and circulation time in vivo.
• Polymer Chemistry: As a monomer or chain extender in the synthesis of functionalized, biodegradable polymers for medical applications.
• Surface Functionalization: For modifying materials like gold nanoparticles, quantum dots, or microarrays to create bioactive interfaces.
• Proteomics Research: In the preparation of affinity tags, crosslinking agents for mass spectrometry, or solid-phase synthesis supports.
• Diagnostic Kit Manufacturing: Used in the production of enzyme conjugates or labeled probes for immunoassays and biosensors.

Basic Information

Product Name	H2N-Peg2-Tbu
CAS No.	756525-95-8
Molecular Formula	C13H27NO5
Molecular Weight	277.36 g/mol
Synonyms	2-[2-(2-Aminoethoxy)ethoxy]acetic acid tert-butyl ester; tert-Butyl 2-[2-(2-aminoethoxy)ethoxy]acetate; Amino-PEG2-t-butyl ester; H2N-PEG2-COOtBu; 2,5,8,11-Tetraoxatridecan-13-oic acid, 13-amino-4,4-dimethyl-13-oxo-, 1,1-dimethylethyl ester; Amino-dPEG®2-t-butyl ester; Amine-PEG2-acid t-butyl ester; Boc-NH-PEG2-COOtBu (common error, note: this is the *tert-butyl* ester, not Boc-protected amine).
EINECS	Contact for details

Quality Control

Our H2N-Peg2-Tbu is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and pharmaceutical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided and can be tailored to meet specific customer or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as specified on the label. Due to its hygroscopic nature, the container should be sealed under an inert atmosphere (e.g., nitrogen or argon) after each use to prevent moisture absorption and degradation. Keep away from strong acids and bases.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR / NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.