Description

α-Naphthoflavone-5,6-Dihydrodiol is a high-purity synthetic dihydrodiol derivative of the flavonoid compound α-naphthoflavone. This compound serves as a critical reference standard and biochemical research tool, particularly in studies of cytochrome P450 metabolism and aryl hydrocarbon receptor (AhR) pathways. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and contract research organizations (CROs) engaged in drug metabolism, toxicology, and environmental health science.

Application

• Biochemical Research Standard: Used as a certified reference material (CRM) for calibrating analytical instruments in metabolic studies.
• Cytochrome P450 Metabolism Studies: Acts as a specific metabolite standard for investigating the metabolic pathways of polycyclic aromatic hydrocarbons (PAHs) and related flavonoids.
• Aryl Hydrocarbon Receptor (AhR) Research: Employed as a tool compound to study AhR activation, signal transduction, and downstream gene expression effects.
• Toxicology and Carcinogenicity Screening: Serves as a key intermediate in research models assessing the bioactivation and detoxification of potential carcinogens.
• Analytical Method Development: Used by QC laboratories to develop and validate HPLC, LC-MS, or GC-MS methods for metabolite detection and quantification.
• Enzyme Kinetics Studies: Applied in vitro to determine kinetic parameters (Km, Vmax) for specific CYP isozymes like CYP1A1 and CYP1B1.

Basic Information

Product Name	α-Naphthoflavone-5,6-Dihydrodiol
CAS No.	78729-84-7
Molecular Formula	C19H14O4
Molecular Weight	306.31 g/mol
Synonyms	5,6-Dihydro-5,6-dihydroxy-α-naphthoflavone; α-Naphthoflavone 5,6-dihydrodiol; 5,6-Dihydroxy-5,6-dihydro-α-naphthoflavone; ANF-5,6-dihydrodiol; Benzo[h]chromen-4-one, 5,6-dihydro-5,6-dihydroxy-2-(1-naphthalenyl)-; 2-(Naphthalen-1-yl)-5,6-dihydroxy-5,6-dihydro-4H-benzo[h]chromen-4-one
EINECS	Contact for details

Quality Control

Our α-Naphthoflavone-5,6-Dihydrodiol is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing results from HPLC, NMR, and MS are provided with every shipment. We support research requiring traceable standards and can supply material characterized for use in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.