Description

3H-Diazirine-3,3-Dicarboxylic Acid Monomethyl Ester Potassium Salt is a versatile and highly reactive diazirine-based photoaffinity labeling reagent. This compound is critical for advanced biochemical research, enabling the covalent cross-linking and identification of biomolecular interaction sites upon photoactivation. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors for applications in proteomics, drug discovery, and molecular biology.

Application

• Photoaffinity Labeling (PAL): A key reagent for mapping protein-ligand, protein-protein, and protein-nucleic acid interactions in complex biological systems.
• Proteomics Research: Used to identify and characterize transient or weak binding sites on target proteins, aiding in the understanding of biological pathways.
• Drug Discovery & Development: Facilitates the study of drug-target engagement and off-target effects by irreversibly tagging active compounds to their biological targets.
• Bioconjugation & Chemical Biology: Serves as a photoreactive handle for the site-specific modification of biomolecules, enabling the creation of novel probes and tools.
• Material Science: Potential use in surface functionalization and the development of photo-crosslinkable polymers and coatings.

Basic Information

Product Name	3H-Diazirine-3,3-Dicarboxylic Acid Monomethyl Ester Potassium Salt
CAS No.	76429-92-0
Molecular Formula	C5H5KN2O4
Molecular Weight	196.21 g/mol
Synonyms	Potassium 3-(Methoxycarbonyl)-3H-diazirine-3-carboxylate; Diazirine-3,3-dicarboxylic Acid Monomethyl Ester Potassium Salt; 3-(Methoxycarbonyl)-3H-diazirine-3-carboxylic Acid Potassium Salt; Methyl Hydrogen Diazirine-3,3-dicarboxylate Potassium Salt; Photoaffinity Labeling Reagent; PAL Reagent; Diazirine Photoprobe; Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Our 3H-Diazirine-3,3-Dicarboxylic Acid Monomethyl Ester Potassium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for assay and purity, NMR for structural confirmation, and Karl Fischer titration for moisture content. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and light-sensitive; handle under an inert atmosphere when possible and minimize exposure to ambient conditions.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.