Description

n-(4-(4-Azidophenylazo)Benzoyl)-3-Aminopropyl-N'-Oxysuccinimide Ester is a specialized heterobifunctional crosslinking reagent featuring both a photoactivatable aryl azide group and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound matters for researchers and manufacturers requiring precise, light-controlled conjugation of biomolecules, enabling the creation of stable covalent bonds under mild UV irradiation. It is essential for professionals in bioconjugation, proteomics research, and advanced materials science who need reliable, high-purity reagents for developing diagnostic assays, affinity labels, and surface modification strategies.

Application

• Bioconjugation and Protein Labeling: For covalently attaching proteins, antibodies, or peptides to other molecules, surfaces, or solid supports via its photoreactive and amine-reactive groups.
• Photoaffinity Labeling: Used in proteomics to "trap" and identify transient protein-protein or protein-ligand interactions upon UV light activation.
• Surface Modification and Immobilization: Functionalizing biosensor chips, microarray slides, or nanoparticles with biomolecules for diagnostic and research applications.
• Polymer and Biomaterial Crosslinking: Incorporating into hydrogel networks or polymer backbones to create photo-crosslinkable, bioactive materials.
• Development of Diagnostic Kits: As a key reagent in the manufacture of ELISA kits, lateral flow assays, and other immunoassay platforms.
• Affinity Chromatography Ligand Synthesis: For immobilizing specific ligands onto chromatography resins for protein purification.

Basic Information

Product Name	n-(4-(4-Azidophenylazo)Benzoyl)-3-Aminopropyl-N'-Oxysuccinimide Ester
CAS No.	72755-63-6
Molecular Formula	C24H20N6O5
Molecular Weight	472.45 g/mol
Synonyms	4-((4-Azidophenyl)diazenyl)benzoic acid 3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl ester; 4-Azidobenzeneazobenzoic Acid NHS Ester; Aryl Azide NHS Ester; Heterobifunctional Crosslinker, NHS-Azide; p-Azidophenylazo Benzoyl NHS Ester; N-Succinimidyl 4-((4-azidophenyl)diazenyl)benzoate-3-aminopropionate; Photoactivatable NHS Ester; Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Our n-(4-(4-Azidophenylazo)Benzoyl)-3-Aminopropyl-N'-Oxysuccinimide Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and functional activity validation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile
Functional Activity	Conforms (by test conjugation)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.