Description

5-(2-Aminoethyl)Dithio-2-Nitrobenzoic Acid is a specialized biochemical reagent featuring a reactive dithiol linker and a chromogenic nitrobenzoic acid moiety. This compound is crucial for applications requiring controlled disulfide bond formation, protein modification, and spectrophotometric detection. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, and diagnostic assay development.

Application

• Bioconjugation Reagent: For site-specific modification of proteins, peptides, and antibodies via thiol-disulfide exchange reactions.
• Proteomics Research: Used in the study of protein structure, function, and interactions through disulfide bond labeling and analysis.
• Enzyme Activity Assays: Serves as a substrate or chromogenic probe for spectrophotometric detection of thiol-containing enzymes.
• Diagnostic Kit Development: A key component in the formulation of colorimetric diagnostic tests and biosensors.
• Crosslinking Agent: For creating stable disulfide-linked complexes in material science and drug delivery systems.
• Pharmaceutical Intermediate: Used in the synthesis of more complex molecules for therapeutic applications.

Basic Information

Product Name	5-(2-Aminoethyl)Dithio-2-Nitrobenzoic Acid
CAS No.	71899-86-0
Molecular Formula	C9H10N2O4S2
Molecular Weight	274.32 g/mol
Synonyms	DTNB-EA; 5,5'-Dithiobis(2-nitrobenzoic acid) 2-aminoethylamide; 2-Aminoethyl 5-(2-aminoethyldisulfanyl)-2-nitrobenzoate; 5-(2-Aminoethyl)disulfanyl-2-nitrobenzoic Acid; N-(2-Aminoethyl)-5-(2-aminoethyldisulfanyl)-2-nitrobenzamide; AEDTNB; Ellman's Reagent derivative
EINECS	Contact for details

Quality Control

Our 5-(2-Aminoethyl)Dithio-2-Nitrobenzoic Acid is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and industrial applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) due to its hygroscopic and easily oxidized nature. Keep the container in a cool, dry, and well-ventilated area, separate from incompatible materials.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.