Description

Mannosyl(3)-n-Acetylglucosamine(2) Diphosphate Dolichol is a critical lipid-linked oligosaccharide (LLO) intermediate in the biosynthesis of N-linked glycoproteins. This compound matters as it serves as the essential precursor for the oligosaccharyltransferase complex, enabling the transfer of glycan chains to nascent polypeptides in the endoplasmic reticulum. Researchers and manufacturers in the fields of glycobiology, biochemistry, and biopharmaceutical development require this high-purity intermediate to study and modulate protein glycosylation pathways, which are vital for protein folding, stability, and function.

Application

• Biochemical Research: As a key substrate for studying the mechanism and kinetics of the oligosaccharyltransferase (OST) enzyme complex.
• Glycobiology Studies: For investigating the biosynthesis, processing, and biological roles of N-linked glycans in cell culture and in vitro systems.
• Enzyme Assay Development: Used in high-throughput screening (HTS) assays to identify inhibitors or activators of glycosylation enzymes for therapeutic discovery.
• Biopharmaceutical R&D: Essential for research into glycosylation engineering to optimize the efficacy and safety profiles of therapeutic proteins, including monoclonal antibodies.
• Metabolic Pathway Analysis: As a tracer or standard in mass spectrometry-based analysis of congenital disorders of glycosylation (CDG) and other metabolic diseases.
• Academic & Institutional Research: A fundamental reagent for laboratories focused on cell biology, molecular biology, and post-translational modification studies.

Basic Information

Product Name	Mannosyl(3)-n-Acetylglucosamine(2) Diphosphate Dolichol
CAS No.	71892-56-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Dolichyl Diphosphate N-Acetylglucosaminyl Mannose; Dolichol-PP-(GlcNAc)2Man3; Lipid-linked Oligosaccharide Intermediate LLO (Man3GlcNAc2-PP-Dol); Dolichyl Pyrophosphate Chitobiosyl Mannose; (Man)3(GlcNAc)2-PP-Dol; Dol-PP-GlcNAc2Man3; N-Acetylglucosaminyl Mannosyl Diphosphodolichol
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research-grade biochemicals. Each batch is characterized using advanced analytical techniques to confirm identity and purity. Certificates of Analysis (COA) detailing purity, identification, and impurity profiles are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to reach ambient temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.