Description

p-Nitrophenyl β-D-N,N',N''-Triacetylchitotriose is a specialized chromogenic substrate designed for the sensitive and specific detection of chitinase and lysozyme activity. This compound matters significantly in biochemical research and diagnostic development, enabling precise enzymatic quantification critical for studying fungal pathogens, insect physiology, and innate immune responses. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and agricultural biotechnology sectors for high-throughput screening and assay development.

Application

• Enzymatic Assay Substrate: Primary substrate for the colorimetric and spectrophotometric determination of endo-chitinase and lysozyme activity.
• Biochemical Research: Used in studies of chitin metabolism, fungal cell wall biosynthesis, and insect development.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify novel antifungal agents or chitinase inhibitors.
• Diagnostic Development: Integral component in diagnostic kits for detecting specific enzyme activities related to certain infections or physiological states.
• Plant Pathology Studies: Used to assay chitinase activity in plants as a marker for disease resistance and defense responses.
• Quality Control in Enzyme Production: Serves as a standard reagent for the quality assurance and potency testing of commercially produced chitinase enzymes.

Basic Information

Product Name	p-Nitrophenyl β-D-N,N',N''-Triacetylchitotriose
CAS No.	7699-38-9
Molecular Formula	C30H41N4O19
Molecular Weight	737.66 g/mol
Synonyms	4-Nitrophenyl N,N',N''-triacetyl-β-D-chitotrioside; pNP-(GlcNAc)3; 4-Nitrophenyl β-D-N,N',N''-triacetylchitotrioside; Chitotriose p-nitrophenyl derivative; PNP-Triacetylchitotriose; 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside; NAG3-pNP
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-N,N',N''-Triacetylchitotriose is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.