Description

3-Hydroxyflunitrazepam is a significant metabolite of the benzodiazepine flunitrazepam, serving as a key analytical reference standard and research chemical. Its primary value lies in its critical role for forensic, toxicological, and pharmaceutical research, enabling precise detection, quantification, and metabolic pathway studies. This compound is essential for laboratories in the pharmaceutical development, clinical diagnostics, and forensic science sectors requiring high-purity reference materials.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the calibration of analytical instruments and validation of testing methods in drug development and quality control.
• Forensic Toxicology: Critical for the identification and quantification of flunitrazepam and its metabolites in biological samples for legal and medical investigations.
• Clinical Research: Employed in studies investigating the pharmacokinetics, metabolism, and detection windows of benzodiazepines in human subjects.
• Metabolite Studies: Serves as a pure standard for researching the metabolic pathways and biotransformation products of flunitrazepam.
• Method Development: Utilized by analytical laboratories to develop and optimize chromatographic (HPLC, GC) and spectroscopic methods for benzodiazepine analysis.
• Regulatory Compliance Testing: Supports testing protocols for anti-doping agencies and workplace drug screening programs requiring high-specificity assays.

Basic Information

Product Name	3-Hydroxyflunitrazepam
CAS No.	67739-71-3
Molecular Formula	C₁₆H₁₂FN₃O₃
Molecular Weight	313.29 g/mol
Synonyms	3-OH-Flunitrazepam; Flunitrazepam 3-Hydroxy Metabolite; 3-Hydroxy-5-(2-fluorophenyl)-1-methyl-7-nitro-1,3-dihydro-2H-1,4-benzodiazepin-2-one; Norflunitrazepam Hydroxy Metabolite; Ro 03-5250-003; 3α-Hydroxyflunitrazepam; 3-Hydroxy Rohypnol Metabolite
EINECS	Contact for details

Quality Control

Our 3-Hydroxyflunitrazepam is manufactured under strict quality management systems to ensure the highest standards of purity and consistency, suitable for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles as determined by advanced chromatographic and spectroscopic methods. We adhere to relevant guidelines for reference standard materials to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption. For long-term storage, consider storing under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Total Impurities	≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.