Description

Dl-N'-Nitrosonornicotine-2,4,5,6-D4 (Pyridine-D4) is a deuterium-labeled isotopologue of the tobacco-specific nitrosamine N'-Nitrosonornicotine (NNN), a key compound in analytical and toxicological research. This stable isotope-labeled standard is essential for achieving high accuracy and precision in quantitative mass spectrometry, enabling reliable detection and measurement of trace-level analytes. It is primarily required by analytical laboratories, contract research organizations (CROs), and academic institutions conducting advanced research in tobacco carcinogenesis, metabolism studies, and regulatory compliance testing.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative reference standard in LC-MS/MS and GC-MS methods for the accurate determination of NNN in complex matrices.
• Tobacco and Smoke Analysis: Critical for testing tobacco products, smokeless tobacco, and cigarette smoke condensate to quantify levels of this potent carcinogen for regulatory and product development purposes.
• Biomedical and Toxicological Research: Facilitates studies on the metabolic pathways, DNA adduct formation, and carcinogenic mechanisms of NNN in in vitro and in vivo models.
• Environmental and Exposure Monitoring: Employed in assays to assess human exposure to tobacco-specific nitrosamines through environmental tobacco smoke or occupational settings.
• Method Development and Validation: Serves as a crucial tool for developing, optimizing, and validating sensitive analytical methods in compliance with guidelines from agencies like the FDA and WHO.
• Quality Control in Reference Material Production: Used in the preparation and certification of certified reference materials (CRMs) for laboratory proficiency testing and quality assurance programs.

Basic Information

Product Name	Dl-N'-Nitrosonornicotine-2,4,5,6-D4 (Pyridine-D4)
CAS No.	66148-19-4
Molecular Formula	C9H8D4N2O
Molecular Weight	192.24 g/mol
Synonyms	NNN-2,4,5,6-d4; N'-Nitrosonornicotine-2,4,5,6-D4; (RS)-N'-Nitrosonornicotine-2,4,5,6-D4; 1-(3-Pyridyl-D4)-1-butanone 1'-(N-methyl-N-nitrosamino); Deuterated NNN; NNN-d4; [2,4,5,6-2H4]-N'-Nitrosonornicotine
EINECS	Contact for details

Quality Control

Every batch of Dl-N'-Nitrosonornicotine-2,4,5,6-D4 is manufactured and analyzed under strict quality protocols to ensure the highest isotopic purity and chemical integrity required for trace analysis. Our products undergo rigorous quality testing, including NMR and mass spectrometry, to confirm isotopic enrichment and chemical purity. Certificates of Analysis (COA) detailing batch-specific results for identity, purity, isotopic abundance, and solvent content are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere to minimize oxidation and ensure long-term stability. The product is light-sensitive and easily oxidized; therefore, aliquoting under controlled conditions is recommended to prevent repeated freeze-thaw cycles and exposure to air.

Specification

Item	Specification
Appearance	Colorless to pale yellow solution or solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98% (chemical purity)
Isotopic Purity	≥ 98 atom % D
Isotopic Enrichment	≥ 99% at designated positions (2,4,5,6-D4)
Solvent (if supplied as solution)	Specified on COA (e.g., Methanol, Acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.