Description

4-Nitrophenyl-α-D-Maltopentaoside is a specialized chromogenic substrate designed for the sensitive and specific detection of α-amylase activity. This compound matters because it provides a reliable, colorimetric readout essential for enzymatic research and diagnostic assay development. It is primarily needed by biochemical researchers, diagnostic kit manufacturers, and quality control laboratories in the food and pharmaceutical industries for quantifying α-amylase levels.

Application

• Enzymatic Assay Development: As a chromogenic substrate for the kinetic and endpoint measurement of α-amylase activity in research settings.
• Clinical Diagnostics: Used in the formulation of reagent kits for the determination of pancreatic and salivary α-amylase in human serum, urine, and other biological fluids.
• Food Industry Analysis: Employed in standardized methods to assess diastatic activity and starch conversion in brewing, baking, and syrup production.
• Pharmaceutical Quality Control: For testing the enzymatic activity of α-amylase used as an active pharmaceutical ingredient or excipient.
• Microbiology Research: A tool for studying α-amylase production and secretion in microorganisms like bacteria and fungi.
• High-Throughput Screening (HTS): Suitable for automated assay systems in drug discovery to identify inhibitors or activators of α-amylase.

Basic Information

Product Name	4-Nitrophenyl-α-D-Maltopentaoside
CAS No.	66068-38-0
Molecular Formula	C35H55NO28
Molecular Weight	937.80 g/mol
Synonyms	4-Nitrophenyl α-D-maltopentaoside; 4-Nitrophenyl-α-maltopentaoside; PNP-α-G5; p-Nitrophenyl-α-D-maltopentaoside; α-4-Nitrophenylmaltopentaoside; 4-Nitrophenyl α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→4)-D-glucopyranoside; NPG5; Amylase substrate PNP-G5
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-α-D-Maltopentaoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which may affect performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	≥ 95.0% (enzymatic)
Water Content (KF)	≤ 2.0%
Solubility	Clear, colorless solution in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.