Description

4-n-Maleimidobenzoic Acid-Nhs is a heterobifunctional crosslinking reagent featuring both an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive maleimide group. This compound is essential for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules, enabling advanced research and development. It is a critical tool for scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production.

Application

• Bioconjugation and Protein Labeling: For creating covalent linkages between amines (via NHS ester) and thiols (via maleimide) to produce protein-protein or protein-small molecule conjugates.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker-payload intermediate for attaching cytotoxic drugs to monoclonal antibodies with precise control.
• Diagnostic Reagent Manufacturing: Used in the production of enzyme-linked immunosorbent assay (ELISA) kits, fluorescent probes, and other diagnostic tools requiring stable biomolecule immobilization.
• Surface Functionalization: For modifying nanoparticles, biosensor chips, or chromatographic media with specific biomolecular capture agents.
• Polymer and Biomaterial Science: As a crosslinker to introduce reactive handles or create functional hydrogels and polymeric scaffolds for biomedical applications.
• Peptide Synthesis and Modification: To introduce maleimide functionality into peptides for subsequent thiol coupling strategies.

Basic Information

Product Name	4-n-Maleimidobenzoic Acid-Nhs
CAS No.	64191-06-6
Molecular Formula	C15H10N2O6
Molecular Weight	314.25 g/mol
Synonyms	4-Maleimidobenzoic Acid N-Hydroxysuccinimide Ester; 4-(N-Maleimidomethyl)benzoic Acid NHS Ester; 4-Maleimidobenzoic Acid NHS Ester; 4-Maleimidobenzoic Acid Succinimidyl Ester; 4-(Maleimidomethyl)benzoic Acid N-Succinimidyl Ester; 4-Maleimidobenzoyl-N-hydroxysuccinimide Ester; MB-NHS; MBS (Note: Often used interchangeably with 3-Maleimidobenzoic Acid NHS Ester, but distinct isomer)
EINECS	Contact for details

Quality Control

Our 4-n-Maleimidobenzoic Acid-Nhs is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our customers' regulatory requirements in pharmaceutical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.