Description

4-(Methylnitrosoamino)-1-(3-Pyridyl)-1-Butanone is a specialized nitrosamine compound of significant interest in biochemical and toxicological research. Its primary value lies in its role as a key reference standard and analytical marker for studying tobacco-specific nitrosamines (TSNAs) and their metabolic pathways. This high-purity chemical is essential for researchers and quality control laboratories in the pharmaceutical, analytical chemistry, and public health sectors who require precise materials for method development, validation, and contamination studies.

Application

• Analytical Reference Standard: Primary use as a certified reference material (CRM) for the accurate quantification of tobacco-specific nitrosamines in various matrices via HPLC, LC-MS, or GC-MS.
• Toxicology and Carcinogenicity Research: Critical for in vitro and in vivo studies investigating the metabolic activation, DNA adduct formation, and carcinogenic mechanisms of nitrosamines.
• Pharmaceutical Impurity Testing: Employed in method development and validation to monitor and control potentially genotoxic nitrosamine impurities (NTTPs) in active pharmaceutical ingredients (APIs) and drug products, ensuring compliance with ICH M7 and regulatory guidelines.
• Environmental and Food Safety Analysis: Used in the development of sensitive analytical methods to detect trace levels of nitrosamine contamination in environmental samples, food products, and consumer goods.
• Biomarker Studies: Serves as a precursor or standard for investigating specific nitrosamine-derived biomarkers in biological fluids for exposure assessment.

Basic Information

Product Name	4-(Methylnitrosoamino)-1-(3-Pyridyl)-1-Butanone
CAS No.	64091-50-5
Molecular Formula	C10H13N3O2
Molecular Weight	207.23 g/mol
Synonyms	NNK; 4-(N-Methyl-N-nitrosamino)-1-(3-pyridinyl)-1-butanone; N-Nitrosonornicotine Ketone; Tobacco-Specific Nitrosamine NNK; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone; 3-(1-Oxo-4-methylnitrosaminobutyl)pyridine; CAS 64091-50-5
EINECS	Contact for details

Quality Control

Our 4-(Methylnitrosoamino)-1-(3-Pyridyl)-1-Butanone is manufactured and handled under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profiles, typically determined by advanced chromatographic techniques. We support compliance with relevant pharmacopeial and analytical standards for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). Due to its light-sensitive nature, handling and aliquoting should be performed under subdued light conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to light brown solid or oil
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95% (Typical 97-99%)
Related Substances (HPLC)	Reported on COA
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.