Description

n-[2-(β-D-Galactopyranosyloxy)-5-Nitrophenyl]Palmitamide is a specialized synthetic glycoside derivative that combines a galactopyranosyl moiety with a nitrophenyl-palmitamide structure. This compound is valued for its role as a chromogenic substrate in enzymatic assays, particularly for detecting and quantifying β-galactosidase activity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in biochemical research, molecular biology, and diagnostic development.

Application

• Enzymatic Substrate for β-Galactosidase: Serves as a key chromogenic reagent in biochemical assays to measure enzyme activity in research and diagnostic applications.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) to study gene regulation and promoter activity.
• Diagnostic Kit Manufacturing: A critical component in the formulation of kits for clinical diagnostics and microbiological testing.
• Cell Biology & Histochemistry: Employed in staining techniques to visualize β-galactosidase expression in tissues or cultured cells.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify modulators of β-galactosidase activity for drug discovery.
• Food & Environmental Microbiology: Applied in tests for bacterial detection (e.g., coliforms) that express β-galactosidase.

Basic Information

Product Name	n-[2-(β-D-Galactopyranosyloxy)-5-Nitrophenyl]Palmitamide
CAS No.	63424-42-0
Molecular Formula	C34H56N2O10
Molecular Weight	652.82 g/mol
Synonyms	5-Nitrophenyl β-D-Galactopyranoside Palmitamide; Galactopyranosyloxy Nitrophenyl Palmitamide; PNPG-Palmitamide; 2-(β-D-Galactopyranosyloxy)-5-nitro-N-pentadecylbenzamide; o-Nitrophenyl-β-D-galactopyranoside palmitamide derivative; Chromogenic Galactosidase Substrate; β-Galactosidase Chromogenic Substrate Analog
EINECS	Contact for details

Quality Control

Our n-[2-(β-D-Galactopyranosyloxy)-5-Nitrophenyl]Palmitamide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (IR, NMR), and residual solvent analysis to ensure it meets the high standards required for sensitive biochemical applications. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.