Description

4-Nitrophenol Sodium Salt Dihydrate is a water-soluble, crystalline organic salt widely used as a key intermediate and analytical reagent. Its primary value lies in its role as a chromogenic substrate for enzyme activity assays, particularly for alkaline phosphatase, making it essential for biochemical research and diagnostics. This compound is critical for manufacturers and research institutions in the pharmaceutical, biotechnology, and chemical synthesis sectors requiring high-purity analytical standards and fine chemical building blocks.

Application

• Enzyme Substrate: Primary chromogenic substrate for colorimetric assays of alkaline phosphatase activity in ELISA and other diagnostic kits.
• Biochemical Research: Essential reagent in life science research for studying enzyme kinetics, inhibition, and cellular signaling pathways.
• Diagnostic Manufacturing: Used in the production of clinical diagnostic reagents and test strips for medical and veterinary applications.
• Chemical Synthesis: Serves as a protected or activated intermediate in the synthesis of more complex dyes, pharmaceuticals, and agrochemicals.
• Photographic Industry: Acts as a chemical component in certain photographic developers and sensitizers.
• Laboratory Standard: Utilized as a pure reference standard for calibration and quality control in analytical laboratories.

Basic Information

Product Name	4-Nitrophenol Sodium Salt Dihydrate
CAS No.	63317-67-9
Molecular Formula	C6H4NNaO3 · 2H2O
Molecular Weight	191.10 g/mol (Anhydrous basis)
Synonyms	p-Nitrophenol Sodium Salt Dihydrate; Sodium p-Nitrophenolate Dihydrate; 4-Nitrophenol Sodium Salt; PNPP Sodium Salt; Sodium 4-Nitrophenoxide Dihydrate; Alkaline Phosphatase Substrate; Sodium p-Nitrophenoxide Dihydrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenol Sodium Salt Dihydrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including identification, purity assay, and impurity profiling to ensure it meets the stringent requirements for biochemical and diagnostic applications. Certificates of Analysis (COA) detailing lot-specific results are provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after use to prevent moisture absorption, which can affect product performance and stability.

Specification

Item	Specification
Appearance	Yellow to yellow-orange crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	15.0 - 18.0 %
pH (5% Solution)	7.0 - 9.0
Absorbance (A400nm)	≤ 0.05 (in buffer)
Solubility	Clear, yellow solution in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.