Description

Neu5Ac Alpha(2-6)Gal Beta(1-4)Glcnac-β-Pnp is a sophisticated synthetic oligosaccharide derivative, specifically a p-nitrophenyl (PNP) glycoside of a sialylated N-acetyllactosamine structure. This compound serves as a critical chromogenic substrate for the detection and kinetic study of sialidases (neuraminidases) and other glycosyl hydrolases. It is essential for researchers and developers in the fields of glycobiology, enzymology, and diagnostic assay development, providing a reliable tool for probing enzyme specificity and activity related to sialic acid metabolism.

Application

• Enzyme Substrate for Sialidases/Neuraminidases: Primary use as a chromogenic substrate for the spectrophotometric assay of α2-6 specific sialidase activity.
• Glycobiology Research: Tool compound for studying sialic acid linkage-specific interactions, carbohydrate-protein recognition, and glycan processing pathways.
• Diagnostic Reagent Development: Key component in developing enzymatic assays for clinical diagnostics, including pathogen detection (e.g., influenza neuraminidase characterization).
• Biochemical Profiling: Used in high-throughput screening (HTS) platforms to identify and characterize inhibitors of glycosidases for therapeutic development.
• Quality Control in Biologics: Employed in the analysis of glycosylation patterns of therapeutic proteins, such as monoclonal antibodies and enzymes.
• Academic & Industrial R&D: Fundamental reagent for investigating cell surface glycans, host-pathogen interactions, and the role of sialylation in disease.

Basic Information

Product Name	Neu5Ac Alpha(2-6)Gal Beta(1-4)Glcnac-β-Pnp
CAS No.	501427-93-6
Molecular Formula	C31H45N3O21
Molecular Weight	795.70 g/mol
Synonyms	5-Acetamido-3,5-dideoxy-D-glycero-α-D-galacto-non-2-ulopyranosylonic acid-(2→6)-β-D-galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranosyl β-p-nitrophenyl glycoside; α2-6 Sialyl N-acetyllactosamine PNP; Neu5Acα2-6Galβ1-4GlcNAcβ-PNP; PNP-α2-6 Sialyl LacNAc; Sialidase Substrate (α2-6 linkage); p-Nitrophenyl 5-acetamido-3,5-dideoxy-D-glycero-α-D-galacto-non-2-ulopyranosylonate-(2→6)-β-D-galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside
EINECS	Contact for details

Quality Control

Every batch of Neu5Ac Alpha(2-6)Gal Beta(1-4)Glcnac-β-Pnp is manufactured and analyzed under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for research reproducibility. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with exacting standards for biochemical reagents. Certificates of Analysis (COA) detailing purity, identity, and assay results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Assay (Enzymatic)	≥ 90% (Functional activity)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.