Description

4-Nitrophenyl2-Acetamido-2-Deoxy-4,6-o-p-Methoxybenzylidene-A-D-Galactopyranoside is a specialized glycoside derivative, primarily utilized as a chromogenic substrate in enzymatic assays. Its core value lies in its role as a synthetic intermediate and a critical reagent for biochemical research, enabling the study of glycosidase and glycosyltransferase activities. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors who require high-purity biochemical tools for diagnostic development and carbohydrate chemistry.

Application

• Enzymatic Substrate: Acts as a chromogenic substrate for the detection and kinetic study of specific glycosidases, such as β-galactosidases, in biochemical assays.
• Pharmaceutical Research: Serves as a key synthetic intermediate in the development of novel carbohydrate-based drugs and glycoconjugates.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for enzyme-linked immunosorbent assays (ELISA) and other clinical chemistry applications.
• Biochemical Tool: Employed in academic and industrial research laboratories for investigating carbohydrate metabolism and enzyme mechanisms.
• Glycobiology Studies: Facilitates research into complex carbohydrate structures, cell surface interactions, and glycosylation pathways.

Basic Information

Product Name	4-Nitrophenyl2-Acetamido-2-Deoxy-4,6-o-p-Methoxybenzylidene-A-D-Galactopyranoside
CAS No.	59868-86-9
Molecular Formula	C22H24N2O10
Molecular Weight	476.44 g/mol
Synonyms	p-Nitrophenyl 2-acetamido-2-deoxy-4,6-O-(p-methoxybenzylidene)-α-D-galactopyranoside; 4-Nitrophenyl 2-acetamido-2-deoxy-4,6-O-(4-methoxybenzylidene)-α-D-galactopyranoside; 4-NP-α-GalNAc(4,6-O-p-methoxybenzylidene); PNP-α-GalNAc(4,6-O-PMB); Chromogenic GalNAc derivative; Glycosidase substrate 59868-86-9; α-D-Galactopyranoside, 4-nitrophenyl 2-(acetylamino)-2-deoxy-4,6-O-[(4-methoxyphenyl)methylene]-
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl2-Acetamido-2-Deoxy-4,6-o-p-Methoxybenzylidene-A-D-Galactopyranoside is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. All products undergo rigorous analytical testing, including HPLC and NMR, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with each shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light) and should be handled under appropriate conditions to maintain stability. For long-term storage, consider desiccation to minimize potential moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.