Description

β-D-Gal-(1->3)-β-D-Glcnac-1->Oc6H4No2-P is a chemically defined, synthetic disaccharide derivative featuring a p-nitrophenyl (PNP) aglycon, making it a valuable chromogenic substrate for enzymatic assays. This compound is specifically engineered for high-precision biochemical research and diagnostic development, offering consistent reactivity and purity. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and diagnostic industries for studying glycosidase and glycosyltransferase activities.

Application

• Enzymatic Substrate: Acts as a chromogenic substrate for the assay of β-galactosidase and related enzymes, where cleavage releases the colored p-nitrophenol for easy spectrophotometric detection.
• Glycobiology Research: Used in studies of carbohydrate-active enzymes (CAZymes), including specificity profiling, kinetic analysis, and inhibitor screening for glycosidases and glycosyltransferases.
• Diagnostic Kit Development: Employed in the formulation of diagnostic reagents and kits for clinical and research laboratories to measure specific enzyme activities linked to genetic disorders or biomarkers.
• Biochemical Standard: Serves as a high-purity reference standard for method validation, quality control, and calibration in analytical laboratories.
• Drug Discovery: Utilized in high-throughput screening (HTS) platforms to identify and characterize novel therapeutic agents that modulate glycosidase activity.

Basic Information

Product Name	β-D-Gal-(1->3)-β-D-Glcnac-1->Oc6H4No2-P
CAS No.	57467-13-7
Molecular Formula	C20H28N2O16P
Molecular Weight	574.42 g/mol
Synonyms	p-Nitrophenyl β-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-β-D-glucopyranoside 6-phosphate; PNP-β-D-Gal-(1→3)-β-D-GlcNAc-6-P; 4-Nitrophenyl 6-O-phosphono-3-O-β-D-galactopyranosyl-2-acetamido-2-deoxy-β-D-glucopyranoside; Galβ1-3GlcNAcβ-PNP 6-phosphate; Chromogenic Galβ1-3GlcNAc substrate; Glycosidase substrate 57467-13-7
EINECS	Contact for details

Quality Control

Every batch of β-D-Gal-(1->3)-β-D-Glcnac-1->Oc6H4No2-P is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency. Our quality system is designed to meet the exacting standards of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identification, and specific tests is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Specific Rotation	Contact for details
Enzymatic Activity	Functional as substrate for β-galactosidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.