Description

n-Acetylglucosaminylpyrophosphoryldolichol is a critical lipid-linked intermediate in the eukaryotic N-linked protein glycosylation pathway. This compound matters as it serves as the essential donor substrate for the first mannosyltransferase in the assembly of the lipid-linked oligosaccharide precursor, directly impacting glycoprotein quality and function. It is primarily needed by researchers in biochemistry and molecular biology studying glycosylation mechanisms, as well as by pharmaceutical developers targeting congenital disorders of glycosylation (CDGs) and other related metabolic diseases.

Application

• Biochemical Research: Essential substrate for in vitro studies of the early steps of the N-glycosylation biosynthesis pathway.
• Enzyme Characterization: Used to assay the activity and kinetics of key enzymes like ALG1 (GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase).
• Drug Discovery: Investigational tool for screening potential inhibitors or modulators of glycosylation enzymes for therapeutic intervention.
• Metabolic Disorder Modeling: Critical reagent for studying Congenital Disorders of Glycosylation (CDGs), particularly those associated with ALG gene defects.
• Glycobiology Standards: Serves as a high-purity reference standard for analytical methods (e.g., HPLC, MS) in glycoconjugate analysis.
• Cell-Free Synthesis Systems: Component in reconstituted systems for the production of defined glycoproteins or glycoengineered products.

Basic Information

Product Name	n-Acetylglucosaminylpyrophosphoryldolichol
CAS No.	56938-89-7
Molecular Formula	C88H147NO14P2 (for Dolichol-20 chain length; varies)
Molecular Weight	~1512.1 g/mol (for Dolichol-20 chain length)
Synonyms	GlcNAc-PP-Dolichol; UDP-GlcNAc:Dolichol-P GlcNAc-1-P Transferase Product; N-Acetyl-D-glucosaminyldiphosphodolichol; Dolichyl Pyrophosphate N-Acetylglucosamine; 2-(Acetylamino)-2-deoxy-D-glucopyranosyl Diphosphate Dolichol Ester; ALG7 Product; Lipid-linked GlcNAc
EINECS	Contact for details

Quality Control

Our n-Acetylglucosaminylpyrophosphoryldolichol is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to rigorous analytical standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, desiccate at low temperature.

Specification

Item	Specification
Appearance	White to off-white solid/film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in chloroform, DMSO, chloroform/methanol/water mixtures

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.