Description

Phenyl-D-Galactopyranoside is a specialized glycoside derivative used as a critical chromogenic and fluorogenic substrate in enzymatic assays. This compound is essential for researchers and manufacturers requiring precise detection and quantification of β-galactosidase activity. It is primarily utilized in the life sciences, diagnostic, and pharmaceutical industries for applications ranging from molecular biology research to high-throughput screening.

Application

• Enzymatic Substrate for β-Galactosidase: Acts as a key chromogenic and fluorogenic substrate in assays to detect and measure β-galactosidase (lacZ) activity in molecular biology and microbiology.
• Molecular Biology Research: Used in cloning (e.g., blue-white screening), gene expression studies, and reporter gene assays to monitor promoter activity and transfection efficiency.
• Diagnostic Reagent Manufacturing: Incorporated into kits and reagents for clinical diagnostics, including tests for enzyme deficiencies and bacterial identification (e.g., E. coli).
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotech drug discovery platforms to identify modulators of gene expression or enzyme function.
• Biochemical Research: Serves as a tool for studying enzyme kinetics, inhibition, and the mechanism of action of glycoside hydrolases.
• Food & Beverage Quality Control: Potential use in enzymatic assays for quality testing in food microbiology.

Basic Information

Product Name	Phenyl-D-Galactopyranoside
CAS No.	56390-15-9
Molecular Formula	C12H16O6
Molecular Weight	256.25 g/mol
Synonyms	Phenyl β-D-Galactopyranoside; Phenyl-β-D-galactoside; β-D-Galactopyranosyloxybenzene; 4-Nitrophenyl-β-D-galactopyranoside (related analog); Phenyl galactoside; Galactosyloxybenzene; o-Nitrophenyl-β-D-galactopyranoside (ONPG, related analog); 6-Chloro-3-indolyl-β-D-galactopyranoside (X-Gal, related analog)
EINECS	Contact for details

Quality Control

Our Phenyl-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	97.0% - 103.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in water or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.