Description

o-Nitrophenyl-α-D-Glucopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of α-glucosidase activity, a key enzyme in carbohydrate metabolism research. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for biochemical analysis and enzyme characterization.

Application

• Biochemical Research: As a specific substrate for the spectrophotometric assay of α-glucosidase enzyme activity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for metabolic disorders like Pompe disease.
• Pharmaceutical Development: Used in high-throughput screening (HTS) to identify and characterize potential α-glucosidase inhibitors for drug discovery.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for α-glucosidase.
• Microbiology & Food Science: Applied in assays to study microbial glycosidase activity and in food quality testing.
• Quality Control (QC): Serves as a reference standard for the calibration and validation of enzymatic assay methods in QC laboratories.

Basic Information

Product Name	o-Nitrophenyl-α-D-Glucopyranoside
CAS No.	56193-44-3
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	o-Nitrophenyl α-D-glucopyranoside; 2-Nitrophenyl α-D-glucopyranoside; ONPG-α; α-ONPG; α-D-Glucopyranoside, 2-nitrophenyl; 2-Nitrophenyl-α-D-glucoside; α-Glucosidase substrate; 4-Nitrophenyl α-D-glucopyranoside (related isomer)
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl-α-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.