Description

3,4-Dichloroisocoumarin is a highly reactive heterocyclic compound that serves as a potent and irreversible serine protease inhibitor. This specificity makes it a critical biochemical tool for studying enzyme mechanisms and regulating proteolytic activity in complex systems. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for drug discovery, proteomics research, and biochemical pathway analysis.

Application

• Biochemical Research: As a potent, mechanism-based inhibitor for studying serine proteases like trypsin, chymotrypsin, and elastase.
• Pharmaceutical Development: Used in target validation and as a lead compound or synthetic intermediate for developing novel therapeutic agents.
• Proteomics & Cell Biology: A key tool for inhibiting specific proteases to elucidate their function in cellular processes, signaling pathways, and disease models.
• Diagnostic Reagent Manufacturing: Incorporated into kits and assays designed to measure or control protease activity in clinical and research settings.
• Enzyme Characterization: Employed to determine kinetic parameters and inhibition constants (Ki) for mechanistic studies of enzyme action.

Basic Information

Product Name	3,4-Dichloroisocoumarin
CAS No.	51050-59-0
Molecular Formula	C9H4Cl2O2
Molecular Weight	215.03 g/mol
Synonyms	3,4-DCI; 3,4-Dichloro-1H-2-benzopyran-1-one; 3,4-Dichloro-2H-1-benzopyran-2-one; NSC 663081; 3,4-Dichlorocoumarin (common misnomer); Serine protease inhibitor 3,4-DCI; DCI
EINECS	Contact for details

Quality Control

Our 3,4-Dichloroisocoumarin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and IR for structural confirmation, and residual solvent analysis. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The compound is light-sensitive; prolonged exposure should be avoided to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	149 - 153 °C
Loss on Drying	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.