Description

Bismarck Brown R is a synthetic cationic azo dye belonging to the diazo class, widely recognized for its staining properties in biological and industrial contexts. Its primary value lies in its strong affinity for nucleic acids and acidic tissue components, making it a critical tool for histological and cytological analysis. This compound is essential for laboratories and manufacturers in the life sciences, textile, and leather industries requiring precise and reliable staining agents. We supply high-purity Bismarck Brown R CAS NO 5421-66-9 to meet the stringent demands of research and production applications globally.

Application

• Histological Staining: Used as a counterstain in combination with other dyes (e.g., hematoxylin) for visualizing connective tissue, cartilage, and mast cell granules in tissue sections.
• Cytological Analysis: Employed in staining procedures for sperm morphology assessment and for highlighting specific cellular structures under microscopic examination.
• Textile Dyeing: Functions as a direct dye for coloring natural fibers like cotton, wool, and silk, as well as leather, providing brown shades.
• Biological Indicator: Serves as a pH indicator and a stain for detecting amyloid deposits in biomedical research.
• Microscopy & Diagnostics: A key component in standardized staining kits and protocols for clinical pathology and diagnostic laboratories.
• Industrial Coloring: Applied in the coloration of paper, wood, and other substrates where a specific brown hue is required.

Basic Information

Product Name	Bismarck Brown R
CAS No.	5421-66-9
Molecular Formula	C18H18N8·2HCl
Molecular Weight	419.31 g/mol (for free base)
Synonyms	Bismarck Brown Y; Basic Brown 1; C.I. 21000; Vesuvin; Phenylene brown; Manchester brown; Leather Brown; Diazol Brown; Azo Brown
EINECS	226-548-6

Quality Control

Our Bismarck Brown R is manufactured under a strict quality management system to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including identification by infrared spectroscopy (IR), purity assay by HPLC, and checks for relevant impurities. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee compliance with your specifications and industry standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Keep away from heat sources and incompatible materials. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark brown to black powder or crystalline solid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95% (Dye content)
Loss on Drying	≤ 5.0 %
Ash Content	≤ 1.0 %
Solubility	Soluble in water, slightly soluble in ethanol
pH (1% Solution)	3.0 - 5.0
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.