Description

4-Nitrophenyl 3,6-Di-o-(α-D-Mannopyranosyl)-β-D-Mannopyranoside is a specialized synthetic trisaccharide derivative featuring a chromogenic 4-nitrophenyl aglycone. This compound matters as a critical enzymatic substrate, enabling precise biochemical research and high-throughput screening. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for studying glycosidase enzyme kinetics and inhibitor development.

Application

• Glycosidase Enzyme Substrate: Used as a chromogenic substrate for the assay and characterization of α-mannosidase and related glycosidase enzymes.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and evaluate potential glycosidase inhibitors for therapeutic development.
• Biochemical Research: Serves as a key tool for investigating enzyme mechanisms, specificity, and kinetics in carbohydrate-active enzyme studies.
• Diagnostic Reagent Development: Utilized in the formulation of diagnostic kits for enzyme deficiency disorders, such as certain lysosomal storage diseases.
• Quality Control in Bioprocessing: Applied in monitoring glycosidase activity during the production and purification of biologics and recombinant proteins.

Basic Information

Product Name	4-Nitrophenyl 3,6-Di-o-(α-D-Mannopyranosyl)-β-D-Mannopyranoside
CAS No.	443346-78-9
Molecular Formula	C24H35NO18
Molecular Weight	625.54 g/mol
Synonyms	p-Nitrophenyl 3,6-Di-O-α-D-mannopyranosyl-β-D-mannopyranoside; 4-Nitrophenyl 3,6-di-O-α-D-mannopyranosyl-β-D-mannopyranoside; 4-NP-α-Man-(1→3)-[α-Man-(1→6)]-β-Man; Trisaccharide substrate for α-mannosidase; Chromogenic α-mannosidase substrate; 4-Nitrophenyl trisaccharide derivative
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 3,6-Di-o-(α-D-Mannopyranosyl)-β-D-Mannopyranoside is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dried basis)
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.