Description

n-Ethyl-N'-Nitro-n-Nitrosoguanidine is a specialized nitrosoguanidine derivative used as a potent alkylating agent and mutagen in biochemical research. This compound is critical for inducing targeted mutations in genetic studies, enabling the exploration of gene function and the development of novel microbial strains. It serves as an essential research tool for molecular biologists, geneticists, and industrial microbiologists working in academic, pharmaceutical, and agricultural biotechnology sectors.

Application

• Mutagenesis Studies: Primary reagent for inducing point mutations in bacterial, yeast, and fungal genomes to study gene function and regulation.
• Strain Development: Used in industrial microbiology to develop improved microbial strains for enhanced production of enzymes, antibiotics, or other metabolites.
• Genetic Research: Employed in forward genetic screens to identify genes involved in specific biological pathways or disease models.
• Carcinogenicity Research: Serves as a model alkylating agent in studies investigating DNA damage, repair mechanisms, and chemical carcinogenesis.
• Biochemical Tool: Utilized in vitro to modify proteins or nucleic acids for structure-function relationship studies.

Basic Information

Product Name	n-Ethyl-N'-Nitro-n-Nitrosoguanidine
CAS No.	4245-77-6
Molecular Formula	C₃H₇N₅O₃
Molecular Weight	161.12 g/mol
Synonyms	1-Ethyl-3-nitro-1-nitrosoguanidine; ENNG; N-Ethyl-N'-nitro-N-nitrosoguanidine; N-Ethyl-N-nitro-N'-nitrosoguanidine; Nitrosoethylnitroguanidine; 1-Ethyl-1-nitroso-3-nitroguanidine; N-Ethyl-N-nitroso-N'-nitroguanidine; NSC 93697
EINECS	224-205-8

Quality Control

Our n-Ethyl-N'-Nitro-n-Nitrosoguanidine is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality standards for research chemicals, ensuring reliable performance in sensitive mutagenesis and genetic studies.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. Due to its light-sensitive and easily oxidized nature, prolonged exposure to light, heat, or air should be avoided. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.