Description

Ninhydrin Hydrate CAS NO 485-47-2 is a versatile and highly sensitive chemical reagent, primarily recognized for its role as a chromogenic agent in analytical chemistry. Its core value lies in its exceptional ability to react with amino acids, peptides, and proteins to produce a distinctive purple color, known as Ruhemann's purple, enabling sensitive detection and quantification. This makes it an indispensable tool for professionals in forensic science for fingerprint development, in biochemical research for amino acid analysis, and in quality control laboratories within the pharmaceutical and food industries.

Application

• Forensic Science: Critical reagent for developing latent fingerprints on porous surfaces like paper, cardboard, and untreated wood.
• Biochemical Analysis: Used in thin-layer chromatography (TLC) and paper chromatography for the detection and quantification of amino acids and amines.
• Protein Sequencing & Peptide Mapping: Employed to visualize peptides and N-terminal amino acids after electrophoresis or chromatography.
• Pharmaceutical Quality Control: Assists in the purity testing of amino acid-based drugs and raw materials.
• Food & Feed Analysis: Applied to determine the free amino acid content and profile in nutritional products.
• Histology & Pathology: Used as a staining agent for proteins in tissue sections.
• Document Examination: Helps in revealing indented writing or alterations on paper documents.
• Research & Development: A fundamental reagent in synthetic organic chemistry for the preparation of various derivatives and in studies of reaction mechanisms.

Basic Information

Product Name	Ninhydrin Hydrate
CAS No.	485-47-2
Molecular Formula	C9H6O4 • H2O
Molecular Weight	178.14 g/mol (Anhydrous: 160.13 g/mol)
Synonyms	1,2,3-Indantrione Monohydrate; 2,2-Dihydroxy-1,3-indanedione; Triketohydrindene Hydrate; Ruhemann's Reagent; Ninhydrin; 1,2,3-Triketo-2-hydroindene Monohydrate; 2,2-Dihydroxyindane-1,3-dione; NSC 403697
EINECS	207-618-1

Quality Control

Our Ninhydrin Hydrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for forensic and analytical applications. Key parameters including assay (purity), melting point, and suitability for fingerprint development are verified. A comprehensive Certificate of Analysis (COA) documenting these results is provided with every shipment to guarantee traceability and compliance with your procedural requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed immediately after use to prevent absorption of atmospheric moisture, which can affect performance and stability.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Melting Point	241°C - 245°C (with decomposition)
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition (Sulfated Ash)	≤ 0.1%
Suitability Test (for Fingerprints)	Passes test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.