Description

N'-Nitrosonornicotine n-B-D-Glucuronide (Mixture Of Diastereomers) X Hydrate is a glucuronide conjugate metabolite of the tobacco-specific nitrosamine N'-Nitrosonornicotine (NNN). This compound is of critical importance in analytical and toxicological research for studying the metabolic fate and detoxification pathways of carcinogenic nitrosamines found in tobacco products. It serves as a high-purity reference standard essential for laboratories focused on biomarker analysis, carcinogen metabolism, and regulatory compliance testing. Primary users include contract research organizations (CROs), academic institutions, and analytical service providers in the pharmaceutical and public health sectors.

Application

• Biomarker Reference Standard: Used as a certified reference material for the accurate quantification of NNN metabolites in biological matrices such as urine, blood, and saliva in human biomonitoring studies.
• Toxicology & Metabolism Research: Critical for in vitro and in vivo studies investigating the Phase II glucuronidation detoxification pathway of N'-Nitrosonornicotine, a key tobacco-specific carcinogen.
• Analytical Method Development & Validation: Serves as a primary standard for calibrating LC-MS/MS, HPLC, and other chromatographic systems in method development for nitrosamine analysis.
• Regulatory Compliance & Safety Testing: Supports testing for tobacco product regulation, enabling labs to assess and verify the levels of harmful and potentially harmful constituents (HPHCs) as required by agencies like the FDA.
• Pharmacokinetic Studies: Employed in ADME (Absorption, Distribution, Metabolism, Excretion) research to understand the clearance mechanisms of nitrosamines.
• Quality Control in Reference Material Production: Used by manufacturers as an intermediate or final product for producing secondary reference standards and quality control materials.

Basic Information

Product Name	N'-Nitrosonornicotine n-B-D-Glucuronide (Mixture Of Diastereomers) X Hydrate
CAS No.	350508-25-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NNN-Glucuronide; N'-Nitrosonornicotine Glucuronide; NNN N-glucuronide; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol N-glucuronide (related metabolite); NNN metabolite glucuronide conjugate; NNAL-O-Glucuronide (structural analog); Tobacco-specific nitrosamine glucuronide; NNN-Gluc
EINECS	Contact for details

Quality Control

Our N'-Nitrosonornicotine n-B-D-Glucuronide is produced under a strict quality management system. Each batch undergoes rigorous identity confirmation and purity analysis using advanced techniques including NMR, HPLC, and mass spectrometry to ensure traceability and reliability for research applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific purity, chromatographic data, and storage recommendations. We adhere to industry best practices for reference standard manufacturing to support GLP-compliant laboratory work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or in a desiccated environment after each use to prevent degradation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area Percent)
Water Content (KF)	≤ 5.0 %
Diastereomer Ratio	Specified on COA
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.