Description

2'-Nitrophenyl Tetra-o-Acetyl-A-D-Glucopyranoside is a specialized glycoside derivative, primarily utilized as a chromogenic substrate in enzymatic assays. This compound matters for its role in the sensitive and specific detection of glycosidase enzyme activity, a critical parameter in biochemical research and diagnostic development. It is needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent industries for applications in enzyme kinetics, inhibitor screening, and assay development.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for α-glucosidase and related enzymes, producing a measurable color change upon hydrolysis.
• Biochemical Research & Assay Development: Used in kinetic studies, enzyme characterization, and the development of high-throughput screening (HTS) assays for drug discovery.
• Diagnostic Reagent Manufacturing: Incorporated into diagnostic kits for the detection of specific enzyme deficiencies or activities in clinical samples.
• Quality Control in Bioprocessing: Employed to monitor glycosidase activity during the production and purification of biologics and other fermentation-derived products.
• Academic Research: Serves as a key reagent in university and institutional labs studying carbohydrate metabolism, enzyme mechanisms, and glycobiology.

Basic Information

Product Name	2'-Nitrophenyl Tetra-o-Acetyl-A-D-Glucopyranoside
CAS No.	35023-96-2
Molecular Formula	C20H23NO12
Molecular Weight	469.40 g/mol
Synonyms	4-Nitrophenyl 2,3,4,6-Tetra-O-acetyl-α-D-glucopyranoside; p-Nitrophenyl Tetra-O-acetyl-α-D-glucopyranoside; α-PNPTAG; 2,3,4,6-Tetra-O-acetyl-α-D-glucopyranosyl 4-Nitrophenyl Ether; 4-Nitrophenyl α-D-Glucopyranoside Tetraacetate; PNP-α-GlcNAc (common abbreviation context); α-4-Nitrophenyl Tetraacetylglucoside
EINECS	Contact for details

Quality Control

Our 2'-Nitrophenyl Tetra-o-Acetyl-A-D-Glucopyranoside is produced and tested under a rigorous quality management system. Each batch is analyzed to ensure high purity and consistency, with specifications aligned with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing identity, purity (by HPLC), and impurity profiles is provided with every shipment to guarantee traceability and support your compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.