Description

n-Methyl-3-Indolyl-β-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection of β-galactosidase activity. This compound is critical for research and diagnostic applications where precise enzymatic visualization is required. It is primarily utilized by biotechnology, pharmaceutical, and academic research laboratories involved in molecular biology, reporter gene assays, and histochemical staining.

Application

• Reporter Gene Assays: Used as a substrate for detecting β-galactosidase (lacZ) gene expression in molecular and cellular biology research.
• Histochemistry & Cytochemistry: Enables the localization of β-galactosidase activity in tissue sections and fixed cells, producing an insoluble colored precipitate.
• Microbiology & Diagnostics: Employed in microbial identification systems and diagnostic kits to detect specific bacterial enzymes.
• Enzyme Kinetics Studies: Serves as a specific substrate for studying the kinetics and inhibition of β-galactosidase enzymes.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify modulators of β-galactosidase activity or related pathways.
• Genetic Engineering & Cloning: Acts as a selection or screening marker in cloning vectors that utilize the lacZ gene for blue-white screening.

Basic Information

Product Name	n-Methyl-3-Indolyl-β-D-Galactopyranoside
CAS No.	207598-26-3
Molecular Formula	C15H19NO6
Molecular Weight	309.32 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, N-Methyl Analogue; Methyl-3-indolyl-β-D-galactoside; X-Gal, N-Methyl Derivative; 3-(β-D-Galactopyranosyloxy)-1-methyl-1H-indole; Galactopyranoside, 3-indolyl, N-methyl-; BCIG, N-Methyl; Chromogenic β-galactosidase substrate (N-methyl)
EINECS	Contact for details

Quality Control

Our n-Methyl-3-Indolyl-β-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and performance consistency for sensitive research applications. Certificates of Analysis (COA) detailing purity, identity, and related substance profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.