Description

Hepes (D18) CAS NO 203805-87-2 is a deuterated analog of the widely used biological buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), where eight hydrogen atoms are replaced by deuterium. This isotopic labeling is critical for applications requiring enhanced stability and reduced signal interference in sensitive analytical techniques. It is an essential reagent for researchers and manufacturers in pharmaceutical development, nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry, where precise molecular tracking and structural analysis are paramount.

Application

• NMR Spectroscopy Solvent & Buffer: Used as a deuterated buffer to maintain pH in NMR studies, minimizing proton background signals for clearer analysis of biological macromolecules.
• Stable Isotope Labeling in Mass Spectrometry: Serves as an internal standard or labeling agent in quantitative proteomics and metabolomics for accurate mass shift detection and quantification.
• Biopharmaceutical Process Development: Employed in deuterium exchange mass spectrometry (HDX-MS) to study protein conformation, dynamics, and interactions during drug discovery.
• Cell Culture & Biochemical Research: Provides a stable, deuterated buffering environment for specialized cell culture experiments and enzymatic assays where standard protonated buffers cause interference.
• Deuterated Reagent Synthesis: Acts as a building block or precursor for the synthesis of more complex deuterated compounds in medicinal chemistry and material science.

Basic Information

Product Name	Hepes (D18)
CAS No.	203805-87-2
Molecular Formula	C8D18N2O4S
Molecular Weight	242.39 g/mol
Synonyms	HEPES-d18; Deuterated HEPES; 4-(2-Hydroxyethyl-d4)-1-piperazine-1,2,2,3,3,5,6,6-d8-ethanesulfonic Acid-d2; 2-[4-(2-Hydroxyethyl-d4)piperazin-1-yl-1,2,2,3,3,5,6,6-d8]ethanesulfonic Acid-d2; D-HEPES; HEPES (Octadeutero); Perdeuterated HEPES Buffer
EINECS	Contact for details

Quality Control

Our Hepes (D18) is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high isotopic purity and chemical integrity, meeting the exacting standards required for advanced research. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as isotopic enrichment, chemical purity, and NMR solvent compatibility.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to prevent absorption of atmospheric moisture, which can affect isotopic purity and stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Purity (D-content)	≥ 98 atom % D
Assay (HPLC)	≥ 98.0%
pH (1M solution in D2O)	7.0 - 7.6
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.