Description

D-Glucose, [2-3H(N)]- is a radiolabeled form of D-glucose, specifically tritiated at the 2-position, with the CAS registry number 22348-49-8. This high-specific-activity compound is an essential tracer for investigating glucose metabolism, transport, and utilization in biological systems. It is a critical reagent for researchers and scientists in the fields of biochemistry, pharmacology, and metabolic disease research, enabling precise tracking and quantification in complex in vitro and in vivo studies.

Application

• Metabolic Pathway Tracing: Used as a radiolabeled tracer to study glycolysis, the pentose phosphate pathway, and gluconeogenesis in cell cultures and tissue samples.
• Glucose Transport Studies: Essential for quantifying the kinetics and regulation of glucose transporter (GLUT) proteins in various cell types, including adipocytes, muscle cells, and neurons.
• Drug Discovery & Pharmacology: Employed in high-throughput screening assays to evaluate the effects of novel therapeutic compounds on cellular glucose uptake and metabolism.
• Diabetes and Obesity Research: A key tool for investigating insulin resistance, β-cell function, and metabolic alterations in models of metabolic syndrome.
• Neuroscience Research: Applied in studies of brain energy metabolism, neuronal activity, and astrocyte-neuron metabolic coupling.
• Autoradiography and Imaging: Suitable for use in tissue section autoradiography to visualize and localize areas of active glucose metabolism.

Basic Information

Product Name	D-Glucose, [2-3H(N)]-
CAS No.	22348-49-8
Molecular Formula	C6H113HO6
Molecular Weight	~183.2 g/mol (for unlabeled D-Glucose)
Synonyms	2-Tritio-D-glucose; [2-3H]Glucose; D-[2-3H]Glucose; Tritiated D-Glucose; 2-T-Deoxyglucose (common misnomer); Glucose-2-t; Radiolabeled Glucose; 3H-Glucose
EINECS	Contact for details

Quality Control

Every batch of D-Glucose, [2-3H(N)]- is manufactured and analyzed under strict quality systems to ensure radiochemical purity, specific activity, and chemical stability. Comprehensive quality control includes verification by HPLC with radiometric detection. A detailed Certificate of Analysis (COA) is provided with each shipment, specifying the exact specific activity (Ci/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent composition to meet the stringent requirements of research and regulatory applications.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as supplied. For long-term storage, keep at -70°C to minimize radiolytic decomposition. This product is strictly light-sensitive (protect from all light exposure) and should be handled in accordance with all local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	10-40 Ci/mmol (370-1480 GBq/mmol)
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Concentration	1 mCi/mL (37 MBq/mL)
Solvent	Ethanol:Water (9:1 v/v)
Identification (IR/NMR)	Conforms to structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.