Description

Bis-Sulfone-Peg4-Dbco is a heterobifunctional crosslinking agent featuring a dibenzocyclooctyne (DBCO) group and a bis-sulfone moiety, connected by a tetraethylene glycol (PEG4) spacer. This compound is specifically engineered for high-efficiency, copper-free click chemistry applications, enabling stable and selective bioconjugation under mild, physiological conditions. It is an essential reagent for researchers and developers in bioconjugation, pharmaceutical development, and advanced materials science, particularly for labeling biomolecules, constructing antibody-drug conjugates (ADCs), and functionalizing surfaces or nanoparticles.

Application

• Bioconjugation and Protein Labeling: Site-specific conjugation of peptides, proteins, and antibodies with fluorophores, drugs, or other functional molecules via strain-promoted alkyne-azide cycloaddition (SPAAC).
• Antibody-Drug Conjugate (ADC) Development: Construction of stable, homogeneous ADCs by linking cytotoxic payloads to monoclonal antibodies through the reactive bis-sulfone group.
• Surface and Nanoparticle Functionalization: Modification of polymers, hydrogels, and nanomaterials (e.g., gold nanoparticles, quantum dots) to introduce bio-recognition elements.
• Proteomics and Biomarker Discovery: Immobilization of bait molecules for affinity purification or pull-down assays to study protein-protein interactions.
• Diagnostic Assay Development: Conjugation of detection probes (enzymes, dyes) to antibodies or antigens for use in ELISA, lateral flow assays, and other diagnostic platforms.
• Polymer and Material Science: Synthesis of functionalized polymers and smart materials with tailored biological activity or responsiveness.

Basic Information

Product Name	Bis-Sulfone-Peg4-Dbco
CAS No.	1854034-70-0
Molecular Formula	C₃₄H₄₄N₂O₁₂S₂
Molecular Weight	724.85 g/mol
Synonyms	DBCO-PEG4-bis-sulfone; Bis-sulfone-PEG4-DBCO; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-{2,2-dimethyl-4-oxo-3,8,11,14-tetraoxa-5,6-dithia-2-azahexadecan-16-yl}carbamate; 1-(1,3-Dioxo-1,3-dihydro-2H-isoindol-2-yl)-3,6,9,12-tetraoxapentadecan-15-yl 4-({4-[(1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethoxy]carbonyl}amino)butyl sulfone; Heterobifunctional PEG crosslinker DBCO-PEG4-bis-sulfone; DBCO-PEG4-Bis-sulfone linker.
EINECS	Contact for details

Quality Control

Our Bis-Sulfone-Peg4-Dbco is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, identity, and consistency. We provide comprehensive Certificates of Analysis (COA) with detailed specifications, including assay by HPLC, confirmation of structure by NMR and mass spectrometry, and quantification of impurities. Our quality commitment supports applications in research and development where reproducibility and reliability are paramount.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.