Description

Tetrazine-PEG5-COOH is a heterobifunctional crosslinker featuring a highly reactive s-tetrazine moiety and a carboxylic acid group, connected by a flexible, hydrophilic polyethylene glycol (PEG) spacer. This compound is specifically engineered for bioorthogonal conjugation applications, enabling rapid and selective labeling of biomolecules under physiological conditions without interfering with native biological processes. It is an essential reagent for researchers and developers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and advanced materials science, where precise and stable linkage is critical.

Application

• Bioorthogonal Labeling: Utilized in Inverse Electron Demand Diels-Alder (IEDDA) reactions with trans-cyclooctene (TCO) or norbornene for rapid, catalyst-free labeling of proteins, antibodies, and live cells.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker-payload component for constructing stable, homogeneous ADCs with controlled drug-to-antibody ratios (DAR).
• Fluorescent Probe Synthesis: Conjugation point for attaching fluorophores (via the COOH group) to tetrazine-based targeting ligands for in vivo imaging and diagnostics.
• Surface Functionalization: Used to modify nanoparticles, biosensors, and other material surfaces with bioactive ligands for targeted delivery and sensing.
• Chemical Biology Tool Development: Enables the study of biomolecular interactions and dynamics through site-specific tagging and pull-down assays.
• Polymer and Hydrogel Modification: Incorporates reactive handles into polymeric materials for creating smart, responsive biomaterials.

Basic Information

Product Name	Tetrazine-PEG5-COOH
CAS No.	1682653-79-7
Molecular Formula	C26H38N4O9
Molecular Weight	550.60 g/mol
Synonyms	3-(1,2,4,5-Tetrazin-3-yl)propanoic acid-PEG5-acid; 1,2,4,5-Tetrazine-3-propionic acid-PEG5-acid; s-Tetrazine-PEG5-acid; TZ-PEG5-COOH; 3-(6-(2,5,8,11,14-Pentaoxahexadecan-16-yloxy)-1,2,4,5-tetrazin-3-yl)propanoic acid; Tetrazine-PEG5-Acid; Tetrazine-Pentaethylene glycol-Carboxylic Acid
EINECS	Contact for details

Quality Control

Our Tetrazine-PEG5-COOH is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and MS for identity verification. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support development under cGMP guidelines for pre-clinical and clinical-stage projects.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to purple solid or oil
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.