Description

3',6'-Bis{[4-(4,4,5,5-Tetramethyl-1,3,2-Dioxaborolan-2-Yl)Oxy]-3H-[Spiro(Isobenzofuran-1,9,-Xanthen]-3-One} Fbbbe is a high-purity, advanced organic intermediate featuring two protected boronic ester functional groups attached to a fluorescent xanthene core. This compound is critical for researchers and manufacturers requiring precise, site-specific conjugation in the development of novel diagnostic and therapeutic agents. It is primarily utilized in the pharmaceutical, biotechnology, and advanced materials sectors for applications such as bioorthogonal labeling and the synthesis of complex molecular probes.

Application

• Bioconjugation & Probe Synthesis: Serves as a key building block for creating fluorescent probes and antibody-drug conjugates (ADCs) via Suzuki-Miyaura cross-coupling reactions.
• Diagnostic Assay Development: Used in the design and manufacture of reagents for fluorescence-based immunoassays, flow cytometry, and in vivo imaging.
• Pharmaceutical Research: Enables the late-stage functionalization of drug candidates and biomolecules for targeted delivery and pharmacokinetic studies.
• Materials Science: Employed in the synthesis of functional polymers and smart materials where the fluorescent properties and boronic acid handles are leveraged.
• Chemical Biology: Facilitates the study of biological processes through bioorthogonal chemistry and the labeling of biomolecules in live cells.

Basic Information

Product Name	3',6'-Bis{[4-(4,4,5,5-Tetramethyl-1,3,2-Dioxaborolan-2-Yl)Oxy]-3H-[Spiro(Isobenzofuran-1,9,-Xanthen]-3-One} Fbbbe
CAS No.	1522117-83-4
Molecular Formula	C46H48B2O10
Molecular Weight	782.48 g/mol
Synonyms	3',6'-Bis(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxy)spiro[isobenzofuran-1,9'-xanthen]-3-one; Fluorescein bis(4-pinacol boronate ester); FBBBE; Fluorescein bisboronic acid pinacol ester; 3',6'-Bis(4-(pinacolatoboryl)oxy)fluorescein; 3',6'-Di(4-boronic acid pinacol ester)fluorescein; 1522117-83-4; Fluorescein-based bis-boronate probe.
EINECS	Contact for details

Quality Control

Every batch of 3',6'-Bis{[4-(4,4,5,5-Tetramethyl-1,3,2-Dioxaborolan-2-Yl)Oxy]-3H-[Spiro(Isobenzofuran-1,9,-Xanthen]-3-One} Fbbbe is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We ensure compliance with the high-purity standards required for pharmaceutical and diagnostic research. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profiles via HPLC, NMR, and MS is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. Due to its hygroscopic (moisture-sensitive) nature, the container should be kept sealed under an inert atmosphere (e.g., argon or nitrogen) when not in use to maintain stability and purity.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.