Description

(S)-n-Nitroso Anatabine-D4 is a high-purity, deuterium-labeled isotopologue of the tobacco alkaloid derivative, n-nitrosoanatabine. This compound is of significant value as a stable isotope-labeled internal standard for precise analytical quantification, particularly in mass spectrometry. It is essential for researchers and quality control laboratories in the pharmaceutical, analytical chemistry, and toxicology sectors who require accurate measurement of nitrosamine impurities or related alkaloid metabolites.

Application

• Internal Standard for LC-MS/MS: Primary use as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for nitrosamine analysis.
• Pharmaceutical Impurity Testing: Critical for method development and validation in detecting and quantifying genotoxic nitrosamine impurities (NTMIs) in active pharmaceutical ingredients (APIs) and finished drug products.
• Tobacco and Smoke Research: Used in studies investigating the formation, metabolism, and biological impact of tobacco-specific nitrosamines (TSNAs).
• Metabolic Pathway Tracing: Employed in deuterium-labeling studies to elucidate metabolic pathways and degradation products of anatabine-related compounds.
• Reference Material for Regulatory Compliance: Supports compliance testing for regulations concerning nitrosamine contaminants (e.g., FDA, EMA guidelines).
• Forensic and Clinical Toxicology: Aids in the accurate identification and measurement of alkaloid derivatives in complex biological matrices.

Basic Information

Product Name	(S)-n-Nitroso Anatabine-D4
CAS No.	1426174-82-4
Molecular Formula	C10H9D4N3O
Molecular Weight	191.24 g/mol
Synonyms	(S)-1-(3-Pyridinyl)-4-(methyl-d3)-1,2,3,6-tetrahydropyridin-2-one-1-nitrosamine; (S)-N-Nitrosoanatabine-d4; N-Nitrosoanatabine (deuterated); N-Nitrosoanatabine-D4; Anatabine, N-nitroso-, labeled with deuterium; TSNA-d4 (specific); Nitrosamine-d4 internal standard
EINECS	Contact for details

Quality Control

Every batch of (S)-n-Nitroso Anatabine-D4 is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data to support its use in regulated environments. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting critical parameters including isotopic purity (D-enrichment), chemical purity by HPLC, and identity confirmation. Our quality commitment ensures the material meets the stringent requirements for use as a certified reference material in analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under inert atmosphere for long-term stability. For short-term use, the material may be stored at 2-8°C. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.