Description

n-Desmethylflunitrazepam-D4 is a stable isotope-labeled analog of a key benzodiazepine metabolite, specifically deuterated at four positions. This compound is essential for providing an internal standard in quantitative mass spectrometry, ensuring the highest accuracy and reliability in analytical results. It is primarily required by forensic, clinical, and pharmaceutical research laboratories for precise drug metabolism studies, toxicological screening, and method validation.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of n-Desmethylflunitrazepam and related benzodiazepines in complex biological matrices.
• Forensic Toxicology: Used in the analysis of blood, urine, and hair samples for drug-facilitated crime investigations and post-mortem toxicology.
• Clinical Research: Enables precise pharmacokinetic and pharmacodynamic studies of flunitrazepam and its metabolites in human subjects.
• Pharmaceutical Development: Supports method development and validation for drug impurity profiling and stability testing.
• Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and ensuring regulatory compliance.
• Metabolite Identification: Aids in the structural elucidation and confirmation of drug metabolites in mass spectrometry-based assays.

Basic Information

Product Name	n-Desmethylflunitrazepam-D4
CAS No.	1397234-19-3
Molecular Formula	C15H8D4FN3O3
Molecular Weight	298.30 g/mol
Synonyms	7-Amino-5-(2-fluorophenyl)-1-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one-D4; 7-Aminoflunitrazepam-D4; Norflunitrazepam-D4; 1,4-Benzodiazepin-2-one, 7-amino-5-(2-fluorophenyl)-1-methyl-1,3-dihydro-, D4-labeled; Flunitrazepam Impurity C-D4; Ro 07-9956-D4; Deuterated n-Desmethylflunitrazepam; [D4]-n-Desmethylflunitrazepam
EINECS	Contact for details

Quality Control

Our n-Desmethylflunitrazepam-D4 is manufactured under strict quality control protocols to ensure isotopic purity and chemical integrity suitable for use as a certified reference standard. Each batch is analyzed by advanced spectroscopic and chromatographic methods, including NMR and HPLC-MS. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98 atom % D), chemical purity, and confirming identity.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.