Description

N’-Nitrosonornicotine-5-Carboxylic Acid is a specialized nitrosamine derivative of nornicotine, identified by CAS NO 1076199-27-3. This compound is of significant interest in analytical chemistry and toxicology research, particularly for the development of reference standards and analytical methods. It serves as a critical intermediate and marker for researchers and quality control laboratories focused on nitrosamine analysis. Primary users include pharmaceutical R&D, analytical testing service providers, and regulatory science organizations investigating tobacco-specific nitrosamines (TSNAs) and related impurities.

Application

• Analytical Reference Standard: Used as a high-purity certified reference material (CRM) for the calibration of analytical instruments like HPLC-MS/MS and GC-MS in nitrosamine testing.
• Pharmaceutical Impurity Profiling: Serves as a key standard for identifying, quantifying, and monitoring process-related nitrosamine impurities in active pharmaceutical ingredients (APIs) and drug products to ensure regulatory compliance.
• Toxicological Research: Employed in metabolic, genotoxicity, and carcinogenicity studies to understand the biological impact and risk assessment of tobacco-specific nitrosamines.
• Method Development & Validation: Critical for developing, optimizing, and validating sensitive analytical methods for trace-level detection of nitrosamines in complex matrices.
• Quality Control Laboratories: Used by QC labs in the pharmaceutical, tobacco, and food industries to establish robust testing protocols for nitrosamine contamination.
• Regulatory Compliance Testing: Essential for testing to meet stringent guidelines from agencies like the FDA, EMA, and ICH regarding nitrosamine limits in consumer products.

Basic Information

Product Name	N’-Nitrosonornicotine-5-Carboxylic Acid
CAS No.	1076199-27-3
Molecular Formula	C10H11N3O3
Molecular Weight	221.21 g/mol
Synonyms	5-Carboxy-N'-nitrosonornicotine; NNN-5-Carboxylic Acid; N'-Nitrosoanatabine-5-carboxylic acid (related); 2-(3-((2S)-1-Methyl-2-pyrrolidinyl)pyridin-5-yl)-N-nitrosoglycine; Tobacco-specific nitrosamine acid metabolite; TSNA carboxylic acid; Nitrosonornicotine acid derivative
EINECS	Contact for details

Quality Control

Our N’-Nitrosonornicotine-5-Carboxylic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous identity confirmation, purity analysis, and impurity profiling using advanced techniques such as HPLC, NMR, and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with batch-specific data, ensuring traceability and compliance with research and analytical standards. Specifications are designed to meet the exacting requirements of reference material production and regulatory testing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under conditions that minimize exposure to ambient light and humidity to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.