Description

n-α-(9-Fluorenylxycarbonyl)-L-Alaninyl-Diazomethane, (3S)-3-Fmoc-Amino-1-Diazo-2-Butanone is a specialized, high-purity amino acid derivative featuring a diazoketone functional group, protected by the Fmoc (9-fluorenylmethoxycarbonyl) group. This compound is a critical building block in modern solid-phase peptide synthesis (SPPS), enabling the controlled introduction of specific amino acid residues under mild conditions. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel peptides, peptidomimetics, and bioconjugates.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key Fmoc-protected amino acid derivative for the controlled, sequential assembly of peptide chains.
• Peptidomimetic Research: Serves as a precursor for creating non-natural amino acid analogs and constrained peptide structures in drug discovery.
• Bioconjugation and Labeling: The reactive diazoketone moiety can be used for site-specific modification of proteins, antibodies, or other biomolecules.
• Combinatorial Chemistry Libraries: Used in the generation of diverse compound libraries for high-throughput screening against biological targets.
• Academic and Pharmaceutical R&D: Essential reagent for investigating peptide structure-activity relationships (SAR) and developing therapeutic candidates.
• Synthesis of Enzyme Inhibitors: Employed in the construction of mechanism-based inhibitors that target specific proteases or enzymes.

Basic Information

Product Name	n-α-(9-Fluorenylxycarbonyl)-L-Alaninyl-Diazomethane, (3S)-3-Fmoc-Amino-1-Diazo-2-Butanone
CAS No.	193954-23-3
Molecular Formula	C20H19N3O4
Molecular Weight	365.39 g/mol
Synonyms	(3S)-3-[(9H-Fluoren-9-ylmethoxy)carbonyl]amino-1-diazo-2-butanone; Fmoc-L-Ala-CHN2; Fmoc-Ala-diazomethane; N-α-Fmoc-L-alaninyl diazomethane; 1-Diazo-3-[(9H-fluoren-9-ylmethoxy)carbonylamino]-2-butanone; Fmoc-Alanine Diazomethyl Ketone; (S)-3-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-1-diazo-2-butanone
EINECS	Contact for details

Quality Control

Our production of this advanced synthetic intermediate adheres to stringent Good Manufacturing Practice (GMP) principles for non-API starting materials. Every batch is subjected to comprehensive analytical characterization, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting critical quality attributes to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dedicated freezer. This compound is strictly light-sensitive (protect from all light exposure) and highly volatile. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress and decomposition.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.