Description

2',4'-Dinitrophenyl 2-Deoxy-2-Fluoro-β-Xylobioside is a specialized fluorinated carbohydrate derivative, primarily utilized as a critical biochemical tool in enzymatic research and diagnostic development. Its core value lies in its role as a chromogenic substrate for detecting and characterizing glycosidase enzymes, particularly xylosidases, due to the 2,4-dinitrophenyl leaving group. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors who require high-purity, reliable substrates for enzyme activity assays and inhibitor screening.

Application

• Enzymology Research: Acts as a chromogenic substrate for the study of β-xylosidase and related glycosidase enzyme kinetics, specificity, and inhibition.
• Diagnostic Reagent Manufacturing: Used in the formulation of kits for the detection and quantification of specific glycosidase activities in clinical and research laboratories.
• Drug Discovery: Serves as a tool compound for screening and evaluating potential glycosidase inhibitors for therapeutic applications.
• Biochemical Assay Development: Employed in the design and optimization of high-throughput screening (HTS) assays for enzyme function.
• Carbohydrate Chemistry Studies: Used as a model compound in research focused on fluorinated sugar analogs and their biochemical interactions.
• Quality Control in Enzyme Production: Applied as a standard substrate to assess the activity and purity of commercially produced glycosidase enzymes.

Basic Information

Item	Detail
Product Name	2',4'-Dinitrophenyl 2-Deoxy-2-Fluoro-β-Xylobioside
CAS No.	156271-23-7
Molecular Formula	C17H21FN2O12
Molecular Weight	464.36 g/mol
Synonyms	2-Deoxy-2-fluoro-β-D-xylobioside 2',4'-dinitrophenyl; DNP-2F-β-Xylobioside; 2,4-Dinitrophenyl 2-deoxy-2-fluoro-β-D-xylobioside; 2'-Deoxy-2'-fluoro-β-xylobioside 2,4-dinitrophenyl ether; Fluorinated xylobioside DNP substrate; β-Xylosidase chromogenic substrate; 156271-23-7; 2,4-DNP-2F-β-Xylob
EINECS	Contact for details

Quality Control

Our 2',4'-Dinitrophenyl 2-Deoxy-2-Fluoro-β-Xylobioside is produced under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the exacting standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.